Hydrochloroauric acid trihydrate (HAuCl₄ 3H₂O, 99.9%) was purchased from Sigma-Aldrich Shanghai Trading Co Ltd, Shanghai, China, while sodium citrate (Na₃C₆H₅O₇ 2H₂O, > 99%) and sodium hydroxide (NaOH, > 98%) were obtained from Shanghai Chemical Co., Shanghai, China. Deionized water (resistance > 18.2 MΩ) was prepared by an ultrapure water system in our laboratory. All chemicals were used as received without any purification.

GNPs were first synthesized from HAuCl₄ solution with gradually increased concentration of the reactant. In detail, 50 ml deionized water in a round-bottom flask was added to 5, 10, 20, 30, 40, and 50 mg chloroauric acid, respectively. After heating to boiling state, 0.3, 0.6, 1.2, 1.8, 2.4, and 3.0 ml sodium citrate solution (50 mg/ml) were rapidly introduced into the flask with drastic stirring, respectively. The mixtures were continuously heated for a certain period till a ruby-red color appeared.

The concentrations of chloroauric acid and sodium citrate in the final mixture were respectively fixed to 2.5 and 5.0 mM, while that of NaOH was changed. The reaction temperature was selected to be boiling state, 85°C and 70°C. For example, 2.0 mL chloroauric acid (25 mM) was mixed with 5.3 to 10.2 mL of 20 mM NaOH solution, followed by adding the calculated volume of water to a total volume of 20 mL. The flask was put into an oil bath at 110°C for 30 min to balance the reaction mixture to 85°C. Then, 0.6 mL sodium citrate solution (50 mg/ml) was rapidly introduced into the flask under vigorous stirring. After different reaction time, samples were taken out for characterization. The reaction at the boiling state and 70°C was similarly performed, respectively.

In the synthesis process of GNPs, a portion of the reaction mixture (0.5 to 1 mL) was taken out from the flask at different reaction time and immediately poured into 9 mL ice-cooled water at 0°C. Such an operation can basically cease the formation process of GNPs due to the low temperature surrounding and the dilution effect, so it was called here as a "sample-frozen" operation. Then, the transmission electron microscopy (TEM) samples were prepared at the earliest time and the ultraviolet-visible (UV-vis) spectra were recorded.

UV-vis spectra were recorded on a U-3010 UV-visible spectrophotometer (Hitachi High-Technologies Co., Tokyo, Japan) to collect the surface plasmon resonance (SPR) information of GNPs, in which the highly concentrated samples were diluted pro rata by deionized water to adapt the measurement limitation. TEM samples were prepared by dropping the diluted gold colloids on carbon-coated copper grids, followed by natural drying; then, the samples were observed on a JEM-2010 microscope (JOEL Ltd, Tokyo, Japan).

In Turkevich's work, the influence of reactant concentration of HAuCl₄ from 0.25 mM to decreased values was studied 25 . Herein, our first effort was taken to prepare GNPs through gradual increase of reactant concentration by the classical citrate method. Aqueous chloroauric acid solution from 0.25 to 2.5 mM was heated to boiling and the four times molar amount of sodium citrate was added, followed by continuously heating for a certain period to get the ruby-red colloids. It was found that the reaction rate was greatly enhanced at high reactant concentration. The optical photos of the obtained samples and diluted samples, as well as the corresponding UV-vis spectra, are shown in Figure 1. The color and the surface plasmon resonance (SPR) peaks of these colloids do not show obvious differences, and no obvious difference is found in the full width at half maximum of these peak profiles. However, TEM images of these GNPs (Figure 2) show that the size polydispersity remarkably varies with the reactant concentration increase although the particle average sizes are all located in a range of 10 to 20 nm. The large size distribution of GNPs at high reactant concentration will limit further applications such as size-related bioassays and well-defined nanoassembly. Moreover, the as-obtained gold colloids from 2.5 mM HAuCl₄ are not stable and become black precipitates after hours; this is partially ascribed to the colloidal instability at high ionic strength.

In recent published work, pH control was reported to produce monodisperse GNPs with low polydispersity 27 43 44 . In our experiments, we found that the increase of the reactant concentration slightly decreased the pH of the final mixture. Thus, we were inspired to add sodium hydroxide (NaOH) into the reaction mixture as a trial to lower particle polydispersity. Then, GNPs were prepared at boiling state with fixed 2.5 mM chloroauric acid and 5 mM sodium citrate (calculated based on the volume of the final mixture). This reduction of the molar ratio of citrate to chloroaurate was applied to decrease the ionic strength in the final colloids. It was found that the reaction rate was reduced as the alkali was added into the reaction system, but precipitates appeared under a high NaOH concentration of 7.8 mM. The color of the obtained colloids was not obviously different from each other (Figure S1 in Additional file 1). Figure 3 shows the TEM images of GNPs synthesized under different NaOH amount from 3.1 to 6.6 mM, and their size distribution was measured from more TEM images as shown below each image. Obviously, the particle size polydispersity was largely decreased with the increase of added NaOH amount. We find that the obtained particles at 5.3 and 6.6 mM NaOH have a narrow size distribution, and the best alkali dosage is 6.6 mM. However, the reaction rate was still found to be too fast to be controlled well, although the alkali's addition could lower it in a certain extent. The time that the color changed to red after adding sodium citrate was still only 1 min in presence of 6.6 mM NaOH, and the reaction flask had to be removed from the oil bath at once, otherwise aggregated particles were obtained (Figure S1 in Additional file 1) possibly due to the kinetic instability 45 . Moreover, at different reaction time, portions of the reaction mixture were taken out and were recorded by UV-vis spectrophotometer. The SPR peaks of these samples (Figure S2 in Additional file 1) show that under the presence of 5.3 and 6.6 mM NaOH, the gold colloids after 1- to 2-min reaction have an SPR peak around 518 nm which corresponds to the uniform colloids. However, at longer reaction time, the SPR peaks are strongly red shifted, indicating an aggregation process in accordance with the TEM results. Therefore, the synthesis time under the boiling state should be no longer than 2 min.

Basically, the chemical reaction rate drastically depends on temperature, so the high rate of nanoparticle formation can be decreased at a low temperature. In this work, the nanoparticle synthesis was therefore performed at 85°C and 70°C with a defined range of NaOH amount. It was found that the formation rate of GNPs slowed as expected at lower temperatures. The color of the colloids obtained at 85°C (Figure S1 in Additional file 1) did not differ from that of those produced under boiling state. TEM images of the synthesized GNPs at 85°C in the presence of different alkali amount were shown in Figure 4 (B1 to B4), including the particle size polydispersity. We could find that GNPs synthesized in presence of 5.5 mM NaOH have an average size of 15 nm with large size distribution, while at a high NaOH concentration, from 6.6 to 8.8 mM, the particle size was slightly decreased to 12 to 13 nm with a narrow distribution. The best GNPs were produced in presence of 7.7 mM NaOH. The higher NaOH dosage of 9.9 mM could only produce purple-color colloid which was not stable and precipitated after hours. The SPR peaks of the gold colloids taken-out from the reaction mixture at different time were also studied by UV-vis spectroscopy (Figure S2 in Additional file 1). We found that the colloidal samples prepared at 6.6 to 8.8 mM NaOH show SPR peaks around 519 nm, and the reaction time should be controlled at 10 to 15 min, although longer reaction time did not cause aggregation.

Similarly, as shown in Figure 4 (C1 to C4), the TEM results of GNPs synthesized at 70°C show the same tendency in particle size and size distribution in presence of different NaOH amount. The dosage of NaOH influences the particle size distribution, and the optimal alkali concentration should be 8.8 mM for the most uniform nanoparticles. The reaction under 9.9 mM NaOH needs a long time heating after citrate addition and produces broadly size distributed GNPs (Figure 4 C4). Optical photos of these gold colloids are shown in the inset of Figure 5. The color of samples prepared under 7.7 and 8.8 mM NaOH is similar, which is slightly different from that of samples prepared at 6.6 and 9.9 mM NaOH. The sample prepared at 5.5 mM NaOH was dark red while that prepared under 11 mM NaOH was cyan due to the aggregation and precipitation of nanoparticles. The SPR peaks (Figure S2 in Additional file 1) of the gold colloids obtained after different reaction times showed that the gold colloids synthesized at optimal conditions (NaOH 7.7 to 8.8 mM) had SPR peaks around 520 nm and the reaction time should be 20 to 25 min.

It can be concluded that uniform GNPs can be synthesized from concentrated gold precursor solution of 2.5 mM based on the citrate reduction by pH and temperature control. The recommended experimental parameters are listed in Table 1. This modified citrate method will largely save energy in the heating stage because of two main reasons. The first is, because the concentration of gold precursor is tenfold compared to the majority of common uses, the usage of only 10% water solvent will save 90% heating energy. Secondly, if the reaction is performed at 70°C or 85°C and room temperature is 25°C, the low temperature reaction will further save 40% or 20% energy, and totally save 94% or 92% heating energy, compared with the dilute concentration and boiling state reaction. Furthermore, it should be noted that the obtained concentrated gold colloids had a good stability, no change was found in the colloid color and the UV-vis absorbance after more than 1-year storage at the room temperature.

Figure 5 displays the pH values of the reaction mixture mixed at room temperature and those as-obtained gold colloids prepared at various conditions. The pH value shows a linear change with respect to the addition of NaOH both before and after the reaction, which is due to the buffer behavior of the sodium citrate and the low alkali dosage. When the reaction was performed at boiling state, the optimal NaOH dosage (6.6 mM) corresponds to pH 6.7. At 85°C, the pH of the best colloids prepared in presence of 7.7 mM NaOH is 6.8, while at 70°C the final pH for the best colloids is 7.5. The pH values of the acceptable GNPs with a narrow size distribution are listed in Table 1. It is found that the pH values for uniform gold colloids are slightly different at different reaction temperatures and a higher pH value is indicated at lower temperature. These pH values are very close to the neutral condition (between 6.5 and 7.5), which is in accordance with the literature 27 .

From the above results, the alkali concentration and the pH value should play a critical role in controlling the size distribution of finally synthesized GNPs. To discover the pH effect on nanoparticle formation, we use a so-called frozen method to cease the nanoparticle growth at different reaction time at 85°C as described in the experimental section and investigate the TEM morphology changes and UV-vis spectra. Three NaOH dosages of 6.0 mM (corresponding to a low pH), 7.8 mM (a medium pH, near the optimal condition), and 9.0 mM (a high pH) were used to prepare reaction-time-dependent samples under different pH conditions. UV-vis spectra and photos (Figure S3 in Additional file 1) of the time-dependent samples can only show the macroscopic changes with time, from which only the difference of the reaction rate can be shown under different pH conditions. The microscopic changes in the process of nanoparticle formation are shown by the TEM images in Figure 6. With the addition of 6.0 mM NaOH, many small particles with about 2 nm in diameter were found after 10-s reaction, and then, the particles grew to 4-nm size at 30 s and about 8-nm particles appeared at 90 s. After 180 s, the formed GNPs did not obviously change their shapes (Figure 6A). In case of 7.8 mM NaOH, similarly, many 3-nm small nanoparticles were found after 30 s (Figure 6B). Then, these small particles grew into large ones of about 10 nm at 210 s, and the final particle size was about 14 nm after 10-min reaction. It should be noticed that these 3-nm small particles continuously exist in the whole particle formation process and even in the final samples (arrow marked). This phenomenon was not found in the low pH case, and it is indicated that the nanoparticle growth step is different at low and medium pH. Thus, the difference in the nanoparticle growth step at low and medium pH might result in the difference of the size polydispersity of the final GNPs. Differently, at high pH (9.5 mM NaOH), both the small particles of about 2 nm and the large particles of about 8 nm (arrow marked) were found after only 30-s reaction (Figure 6C). This is obviously different from the low pH conditions (6.0 and 7.8 mM NaOH) and might imply a different nucleation or coagulation step in the nanoparticle formation at high pH which causes the enlargement of the size distribution. Anyway, the nanoparticle formation process at low or high pH is different from that at mediate pH either in the final nanoparticle growth step or in the beginning nucleation/coagulation step. Therefore, the pH influence on the size distribution of GNPs factually reveals the different formation mechanism of GNPs at different pH conditions as mentioned in the literatures 44 46 47 48 49 .