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Open Access Nano Express

Vertical nanowire probes for intracellular signaling of living cells

Ki-Young Lee12, Ilsoo Kim1, So-Eun Kim1, Du-Won Jeong3, Ju-Jin Kim3, Hyewhon Rhim4, Jae-Pyeong Ahn5, Seung-Han Park6 and Heon-Jin Choi1*

Author Affiliations

1 Department of Materials Science and Engineering, Yonsei University, Seoul 120-749, Republic of Korea

2 Spin Convergence Research Center, Korea Institute of Science and Technology, Seoul 139-791, Republic of Korea

3 Department of Physics, Chonbuk National University, Jeonju 561-756, Republic of Korea

4 Center for Chemoinformatics Research Center, Korea Institute of Science and Technology, Seoul 139-791, Republic of Korea

5 Advanced Analysis Center, Korea Institute of Science and Technology, Seoul 139-791, Republic of Korea

6 Department of Physics, Yonsei University, Seoul 120-749, Republic of Korea

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Nanoscale Research Letters 2014, 9:56  doi:10.1186/1556-276X-9-56

Published: 3 February 2014

Abstract

The single living cell action potential was measured in an intracellular mode by using a vertical nanoelectrode. For intracellular interfacing, Si nanowires were vertically grown in a controlled manner, and optimum conditions, such as diameter, length, and nanowire density, were determined by culturing cells on the nanowires. Vertical nanowire probes were then fabricated with a complimentary metal-oxide-semiconductor (CMOS) process including sequential deposition of the passivation and electrode layers on the nanowires, and a subsequent partial etching process. The fabricated nanowire probes had an approximately 60-nm diameter and were intracellular. These probes interfaced with a GH3 cell and measured the spontaneous action potential. It successfully measured the action potential, which rapidly reached a steady state with average peak amplitude of approximately 10 mV, duration of approximately 140 ms, and period of 0.9 Hz.

Keywords:
Silicon nanowire; Intracellular interfacing; Living cell; Nanowire probe; Action potential