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In vitro performance of lipid-PLGA hybrid nanoparticles as an antigen delivery system: lipid composition matters

Yun Hu1, Marion Ehrich2, Kristel Fuhrman3 and Chenming Zhang1*

Author Affiliations

1 Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA

2 Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA 24061, USA

3 Veterinary Medicine Experiment Station, Virginia Tech, Blacksburg, VA 24061, USA

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Nanoscale Research Letters 2014, 9:434  doi:10.1186/1556-276X-9-434

Published: 27 August 2014


Due to the many beneficial properties combined from both poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and liposomes, lipid-PLGA hybrid NPs have been intensively studied as cancer drug delivery systems, bio-imaging agent carriers, as well as antigen delivery vehicles. However, the impact of lipid composition on the performance of lipid-PLGA hybrid NPs as a delivery system has not been well investigated. In this study, the influence of lipid composition on the stability of the hybrid NPs and in vitro antigen release from NPs under different conditions was examined. The uptake of hybrid NPs with various surface charges by dendritic cells (DCs) was carefully studied. The results showed that PLGA NPs enveloped by a lipid shell with more positive surface charges could improve the stability of the hybrid NPs, enable better controlled release of antigens encapsulated in PLGA NPs, as well as enhance uptake of NPs by DC.

Hybrid NP; Lipid composition; PLGA; Surface charge; Vaccine; Antigen delivery