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Open Access Nano Express

Immobilization of peroxidase enzyme onto the porous silicon structure for enhancing its activity and stability

Padmavati Sahare1, Marcela Ayala2, Rafael Vazquez-Duhalt3 and Vivechana Agrawal1*

Author Affiliations

1 Centro de Investigacion en Ingenieria y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Cuernavaca, Morelos 62209, México

2 Instituto de Biotecnología Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos 62250, México

3 Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Km 107 Carretera Tijuana-Ensenada, Apdo Postal 14, CP. 22800 Ensenada, B.C., México

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Nanoscale Research Letters 2014, 9:409  doi:10.1186/1556-276X-9-409

Published: 21 August 2014

Abstract

In this work, a commercial peroxidase was immobilized onto porous silicon (PS) support functionalized with 3-aminopropyldiethoxysilane (APDES) and the performance of the obtained catalytic microreactor was studied. The immobilization steps were monitored and the activity of the immobilized enzyme in the PS pores was spectrophotometrically determined. The enzyme immobilization in porous silicon has demonstrated its potential as highly efficient enzymatic reactor. The effect of a polar organic solvent (acetonitrile) and the temperature (up to 50°C) on the activity and stability of the biocatalytic microreactor were studied. After 2-h incubation in organic solvent, the microreactor retained 80% of its initial activity in contrast to the system with free soluble peroxidase that lost 95% of its activity in the same period of time. Peroxidase immobilized into the spaces of the porous silicon support would be perspective for applications in treatments for environmental security such as removal of leached dye in textile industry or in treatment of different industrial effluents. The system can be also applied in the field of biomedicine.

Keywords:
Porous silicon; Peroxidase; Immobilization; Microreactors