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The combined toxicological effects of titanium dioxide nanoparticles and bisphenol A on zebrafish embryos

Jun Yan, Bencheng Lin, Chuanlu Hu, Huashan Zhang, Zhiqing Lin and Zhuge Xi*

Author Affiliations

Tianjin Institute of Health and Environmental Medicine, #55 Munan Road, Heping District, Tianjin 300050, China

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Nanoscale Research Letters 2014, 9:406  doi:10.1186/1556-276X-9-406

Published: 20 August 2014


Environmental pollutants co-exist and exhibit interaction effects that are different from those associated with a single pollutant. As one of the more commonly manufactured nanomaterials, titanium dioxide nanoparticles (TiO2-NPs) are most likely to bind to other contaminants in water. In this paper, we aimed to study the combined toxicological effects of TiO2-NPs and bisphenol A (BPA) on organism. First, in vitro adsorption experiments were conducted to determine the adsorptive interaction between TiO2-NPs and BPA. Second, zebrafish embryo toxicity tests were performed to monitor for changes in the toxicological effects associated with the two chemicals. The study results demonstrated that adsorptive interactions exist between the two chemicals and increased toxicity effects which included an advanced toxicological effect time, decreased survival, increased morphological abnormalities, and delayed embryo hatching. Also, we suggest that the mode of combined action has a synergistic effect. Based on this, we postulate that concomitant exposure to TiO2-NPs and BPA increased BPA bioavailability and uptake into cells and organisms. Further studies are required to understand the mechanisms of interactions of this mixture.

TiO2-NPs; BPA; Combined toxicological effects; Zebrafish embryo; Adsorption