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Open Access Nano Express

Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability

Monica Terracciano12, Ilaria Rea1, Luca De Stefano1*, Ivo Rendina1, Giorgia Oliviero2, Fabrizia Nici2, Stefano D'Errico2, Gennaro Piccialli2 and Nicola Borbone2

Author Affiliations

1 Institute for Microelectronics and Microsystems, National Council of Research, Naples 80131, Italy

2 Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy

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Nanoscale Research Letters 2014, 9:317  doi:10.1186/1556-276X-9-317

Published: 25 June 2014

Abstract

Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.

Keywords:
Mesoporous silicon functionalization; DNA synthesis; Deprotection conditions; Surface stability