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Open Access Nano Express

Optimization on condition of epigallocatechin-3-gallate (EGCG) nanoliposomes by response surface methodology and cellular uptake studies in Caco-2 cells

Xiaobo Luo1, Rongfa Guan1*, Xiaoqiang Chen2*, Miao Tao3, Jieqing Ma1 and Jin Zhao1

Author Affiliations

1 Zhejiang Provincial Engineering Laboratory of Quality Controlling Technology and Instrumentation for Marine Food, China Jiliang University, XueYuan Road 258#, 310018 Hangzhou, People's Republic of China

2 Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Lizhi Road, 430068 Wuhan, China

3 Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, People's Republic of China

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Nanoscale Research Letters 2014, 9:291  doi:10.1186/1556-276X-9-291

Published: 10 June 2014


The major component in green tea polyphenols, epigallocatechin-3-gallate (EGCG), has been demonstrated to prevent carcinogenesis. To improve the effectiveness of EGCG, liposomes were used as a carrier in this study. Reverse-phase evaporation method besides response surface methodology is a simple, rapid, and beneficial approach for liposome preparation and optimization. The optimal preparation conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 4.00, EGCG concentration of 4.88 mg/mL, Tween 80 concentration of 1.08 mg/mL, and rotary evaporation temperature of 34.51°C. Under these conditions, the experimental encapsulation efficiency and size of EGCG nanoliposomes were 85.79% ± 1.65% and 180 nm ± 4 nm, which were close with the predicted value. The malondialdehyde value and the release test in vitro indicated that the prepared EGCG nanoliposomes were stable and suitable for more widespread application. Furthermore, compared with free EGCG, encapsulation of EGCG enhanced its inhibitory effect on tumor cell viability at higher concentrations.

EGCG; Nanoliposomes; Optimization; Response surface methodology; Stability; Cellular uptake