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Open Access Nano Express

BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer

Chao Li1, Yang Ji2, Can Wang3, Shujing Liang1, Fei Pan1, Chunlei Zhang1, Feng Chen1, Hualin Fu1, Kan Wang1 and Daxiang Cui1*

Author Affiliations

1 Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Research Institute of Micro/Nano Science and Technology, Department of Instrument Science and Engineering, School of Electronics and Information, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China

2 Department of Imaging Center, Affiliated Hospital of Xi'an Medical University, Xi'an 710077, People's Republic of China

3 Department of Orthopedics, Xiangyan Hospital of Central South University, Changsha 410008, People's Republic of China

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Nanoscale Research Letters 2014, 9:244  doi:10.1186/1556-276X-9-244

Published: 19 May 2014

Abstract

Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like alkyl chains and multiple carboxyl groups, and then used the prepared amphiphilic polymer to modify QDs. The resultant amphiphilic polymer engineered QDs (PQDs) were conjugated with BRCAA1 and Her2 monoclonal antibody, and prepared BRCAA1 antibody- and Her2 antibody-conjugated QDs were used for in vitro MGC803 cell labeling and in vivo targeted imaging of gastric cancer cells. Results showed that the PQDs exhibited good water solubility, strong photoluminescence (PL) intensity, and good biocompatibility. BRCAA1 antibody- and Her2 antibody-conjugated QD nanoprobes successfully realized targeted imaging of in vivo gastric cancer MGC803 cells. In conclusion, BRCAA1 antibody- and Her2 antibody-conjugated PQDs have great potential in applications such as single cell labeling and in vivo tracking, and targeted imaging and therapeutic effects' evaluation of in vivo early gastric cancer cells in the near future.

Keywords:
CdSe/ZnS quantum dots (QDs); Amphiphilic polymer; BRCAA1 antibody; Her2 antibody; Cytotoxicity; Targeted imaging; Gastric cancer cell