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Study on antibacterial activity of silver nanoparticles synthesized by gamma irradiation method using different stabilizers

Dang Van Phu1, Le Anh Quoc1, Nguyen Ngoc Duy1, Nguyen Thi Kim Lan1, Bui Duy Du2, Le Quang Luan3 and Nguyen Quoc Hien1*

Author Affiliations

1 Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, 202A Street 11, Linh Xuan Ward, Thu Duc District, Ho Chi Minh City, Vietnam

2 Institute of Applied Material Science, Vietnam Academy of Science and Technology, 1 Mac Dinh Chi Street, 1 District, Ho Chi Minh City, Vietnam

3 Center for Nuclear Technique, Vietnam Atomic Energy Institute, 217 Nguyen Trai Street, 1 District, Ho Chi Minh City, Vietnam

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Nanoscale Research Letters 2014, 9:162  doi:10.1186/1556-276X-9-162

Published: 4 April 2014


Colloidal solutions of silver nanoparticles (AgNPs) were synthesized by gamma Co-60 irradiation using different stabilizers, namely polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), alginate, and sericin. The particle size measured from TEM images was 4.3, 6.1, 7.6, and 10.2 nm for AgNPs/PVP, AgNPs/PVA, AgNPs/alginate, and AgNPs/sericin, respectively. The influence of different stabilizers on the antibacterial activity of AgNPs was investigated. Results showed that AgNPs/alginate exhibited the highest antibacterial activity against Escherichia coli (E. coli) among the as-synthesized AgNPs. Handwash solution has been prepared using Na lauryl sulfate as surfactant, hydroxyethyl cellulose as binder, and 15 mg/L of AgNPs/alginate as antimicrobial agent. The obtained results on the antibacterial test of handwash for the dilution to 3 mg AgNPs/L showed that the antibacterial efficiency against E. coli was of 74.6%, 89.8%, and 99.0% for the contacted time of 1, 3, and 5 min, respectively. Thus, due to the biocompatibility of alginate extracted from seaweed and highly antimicrobial activity of AgNPs synthesized by gamma Co-60 irradiation, AgNPs/alginate is promising to use as an antimicrobial agent in biomedicine, cosmetic, and in other fields.

Silver nanoparticles; Gamma irradiation; Antibacterial; E. coli; Handwash