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Open Access Nano Express

Grafting of bovine serum albumin proteins on plasma-modified polymers for potential application in tissue engineering

Nikola Slepičková Kasálková1*, Petr Slepička1, Zdeňka Kolská2, Petra Hodačová3, Štěpánka Kučková3 and Václav Švorčík1

Author Affiliations

1 Department of Solid State Engineering, Institute of Chemical Technology Prague, Technicka 5, Prague 166 28, Czech Republic

2 Faculty of Science, J. E. Purkyne University, Ceske Mladeze 8, Usti nad Labem 400 96, Czech Republic

3 Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technicka 5, Prague 166 28, Czech Republic

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Nanoscale Research Letters 2014, 9:161  doi:10.1186/1556-276X-9-161

Published: 4 April 2014

Abstract

In this work, an influence of bovine serum albumin proteins grafting on the surface properties of plasma-treated polyethylene and poly-L-lactic acid was studied. The interaction of the vascular smooth muscle cells with the modified polymer surface was determined. The surface properties were characterized by X-ray photoelectron spectroscopy, atomic force microscopy, nano-LC-ESI-Q-TOF mass spectrometry, electrokinetic analysis, and goniometry. One of the motivations for this work is the idea that by the interaction of the cell with substrate surface, the proteins will form an interlayer between the cell and the substrate. It was proven that when interacting with the plasma-treated high-density polyethylene and poly-L-lactic acid, the bovine serum albumin protein is grafted on the polymer surface. Since the proteins are bonded to the substrate surface, they can stimulate cell adhesion and proliferation.

Keywords:
Polymers; Plasma treatments; Protein grafting; Surface characterization; Cell interaction