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Optimisation of the synthesis of vancomycin-selective molecularly imprinted polymer nanoparticles using automatic photoreactor

Kateryna Muzyka1*, Khalku Karim2, Antonio Guerreiro2, Alessandro Poma2 and Sergey Piletsky2

Author Affiliations

1 Kharkiv National University of RadioElectronics, Lenin Ave. 14, Kharkiv 61166, Ukraine

2 Chemistry Department, College of Science and Engineering, University of Leicester, Leicester LE1 7RH, UK

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Nanoscale Research Letters 2014, 9:154  doi:10.1186/1556-276X-9-154

Published: 31 March 2014


A novel optimized protocol for solid-state synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) with specificity for antibiotic vancomycin is described. The experimental objective was optimization of the synthesis parameters (factors) affecting the yield of obtained nanoparticles which have been synthesized using the first prototype of an automated solid-phase synthesizer. Applications of experimental design (or design of experiments) in optimization of nanoMIP yield were carried out using MODDE 9.0 software. The factors chosen in the model were the amount of functional monomers in the polymerization mixture, irradiation time, temperature during polymerization, and elution temperature. In general, it could be concluded that the irradiation time is the most important and the temperature was the least important factor which influences the yield of nanoparticles. Overall, the response surface methodology proved to be an effective tool in reducing time required for optimization of complex experimental conditions.

Design of experiments; Molecularly imprinted polymer; Nanoparticles; Immuno-like assay; Automatic photoreactor; Optimization; MODDE