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Capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection

Hao Yang1, Min Deng2, Shan Ga1, Shouhui Chen2, Lin Kang1, Junhong Wang1, Wenwen Xin1, Tao Zhang1, Zherong You1, Yuan An1, Jinglin Wang1* and Daxiang Cui2*

Author Affiliations

1 State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 20 Dongda Street Fengtai District, Beijing 100071, People's Republic of China

2 Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, People's Republic of China

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Nanoscale Research Letters 2014, 9:138  doi:10.1186/1556-276X-9-138

Published: 24 March 2014


Herein, we firstly demonstrate the design and the proof-of-concept use of a capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection. The micropillar array substrate was etched and coated with a gold film by microelectromechanical systems (MEMS) process to integrate into a lateral flow test strip. The detection of abrin solutions of various concentrations was performed by the as-prepared microfluidic chip. It was shown that the correlation between the abrin concentration and SERS signal was found to be linear within the range of 0.1 ng/mL to 1 μg/mL with a limit of detection of 0.1 ng/mL. Our microfluidic chip design enhanced the operability of SERS-based immunodiagnostic techniques, significantly reducing the complication and cost of preparation as compared to previous SERS-based works. Meanwhile, this design proved the superiority to conventional lateral flow test strips in respect of both sensitivity and quantitation and showed great potential in the diagnosis and treatment for abrin poisoning as well as on-site screening of abrin-spiked materials.

Capillary force; Microfluidic chip; SERS; Abrin; Phytotoxin