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Open Access Nano Express

Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors

Margaryta K Shelyakina15, Oleksandr O Soldatkin12*, Valentyna M Arkhypova1, Berna O Kasap3, Burcu Akata34 and Sergei V Dzyadevych12

Author Affiliations

1 Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., Kyiv 03680, Ukraine

2 Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., Kyiv 01003, Ukraine

3 Micro and Nanotechnology Department, Middle East Technical University, Ankara 06531, Turkey

4 Central Laboratory, Middle East Technical University, Ankara 06531, Turkey

5 Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz 55128, Germany

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Nanoscale Research Letters 2014, 9:124  doi:10.1186/1556-276X-9-124

Published: 17 March 2014

Abstract

A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.

Keywords:
Biosensor; Urease; Silicalite; Zeolite; Nano beta; Nano L; pH-sensitive field-effect transistor