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Antiviral activity of silver nanoparticle/chitosan composites against H1N1 influenza A virus

Yasutaka Mori12, Takeshi Ono3, Yasushi Miyahira3, Vinh Quang Nguyen4, Takemi Matsui4 and Masayuki Ishihara2*

Author affiliations

1 Third Division, Aeromedical Laboratory, Japan Air Self-Defense Force, 2-3 Inariyama, Sayama, Saitama, 350-1324, Japan

2 Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

3 Department of Global infectious Diseases and Tropical Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

4 Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino-shi, Tokyo, 191-0065, Japan

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Citation and License

Nanoscale Research Letters 2013, 8:93  doi:10.1186/1556-276X-8-93

Published: 20 February 2013


Silver nanoparticle (Ag NP)/chitosan (Ch) composites with antiviral activity against H1N1 influenza A virus were prepared. The Ag NP/Ch composites were obtained as yellow or brown floc-like powders following reaction at room temperature in aqueous medium. Ag NPs (3.5, 6.5, and 12.9 nm average diameters) were embedded into the chitosan matrix without aggregation or size alternation. The antiviral activity of the Ag NP/Ch composites was evaluated by comparing the TCID50 ratio of viral suspensions treated with the composites to untreated suspensions. For all sizes of Ag NPs tested, antiviral activity against H1N1 influenza A virus increased as the concentration of Ag NPs increased; chitosan alone exhibited no antiviral activity. Size dependence of the Ag NPs on antiviral activity was also observed: antiviral activity was generally stronger with smaller Ag NPs in the composites. These results indicate that Ag NP/Ch composites interacting with viruses exhibit antiviral activity.

Organic/metal nanocomposites; Biomass materials; Antimicrobial materials; Polysaccharides; Nanotoxicity