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Insulin-producing cells could not mimic the physiological regulation of insulin secretion performed by pancreatic beta cells

Qiping Shi1, Simin Luo1, Haiying Jia1, Lie Feng1, Xiaohua Lu1, Lixin Zhou2 and Jiye Cai2*

Author Affiliations

1 The First Affiliated Hospital, Jinan University, Guangzhou 510632, China

2 Institute for Nano-Chemistry, Department of Chemistry, Jinan University, Guangzhou 510632, China

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Nanoscale Research Letters 2013, 8:90  doi:10.1186/1556-276X-8-90

Published: 19 February 2013

Abstract

Objective

The aim of this study was to compare the difference between insulin-producing cells (IPCs) and normal human pancreatic beta cells both in physiological function and morphological features in cellular level.

Methods

The levels of insulin secretion were measured by enzyme-linked immunosorbent assay. The insulin gene expression was determined by real-time quantitative polymerase chain reaction. The morphological features were detected by atomic force microscopy (AFM) and laser confocal scanning microscopy.

Results

IPCs and normal human pancreatic beta cells were similar to each other under the observation in AFM with the porous structure features in the cytoplasm. Both number of membrane particle size and average roughness of normal human beta cells were higher than those of IPCs.

Conclusions

Our results firstly revealed that the cellular ultrastructure of IPCs was closer to that of normal human pancreatic beta cells, but they still could not mimic the physiological regulation of insulin secretion performed by pancreatic beta cells.

Keywords:
Insulin-producing cells; Human normal pancreatic beta cells; Atomic force microscopy