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Open Access Nano Express

DNA molecule stretching through thermo-electrophoresis and thermal convection in a heated converging-diverging microchannel

Shou-Shing Hsieh*, Jyun-Hong Chen and Cheng-Fung Tsai

Author Affiliations

Department of Mechanical and Electromechanical Engineering, National Sun Yat-Sen University, Kaohsiung, 80424, TaiwanRepublic of China

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Nanoscale Research Letters 2013, 8:87  doi:10.1186/1556-276X-8-87

Published: 18 February 2013

Abstract

A novel DNA molecule stretching technique is developed and tested herein. Through a heated converging-diverging microchannel, thermal convection and thermophoresis induced by regional heating are shown to significantly elongate single DNA molecules; they are visualized via a confocal laser scanning microscopy. In addition, electrophoretic stretching is also implemented to examine the hybrid effect on the conformation and dynamics of single DNA molecules. The physical properties of the DNA molecules are secured via experimental measurements.

Keywords:
single DNA molecule stretching; CLSM; thermo-electrophoresis; converging–diverging microchannel