gp100 AuNVs ELISPOT results for conjugation time optimization and comparison of the two-step and one-step methods. (A) The DC-to-pmel-1 splenocyte ELISPOT results for the gp100 AuNVs at different conjugation times. The 1-h method AuNVs gave the most optimal stimulation results between the various incubation times (single asterisk denotes p < 0.05). (B) The DC-to-pmel-1 splenocyte ELISPOT results for a comparison of the two-step and one-step method AuNV (double asterisk denotes p < 0.01).
Lin et al. Nanoscale Research Letters 2013 8:72 doi:10.1186/1556-276X-8-72