CLSM images of Caco-2 cells after 2-h incubation with coumarin-6-loaded 5% thiolated chitosan-modified PLA-PCL-TPGS nanoparticles at 37.0°C. The cells were stained by DAPI (blue), and the coumarin-6-loaded nanoparticles are green. The cellular uptake was visualized by overlaying images obtained by EGFP filter and DAPI filter: left image from EGFP channel (A), center image from DAPI channel (B), right image from combined EGFP channel and DAPI channel (C).
Jiang et al. Nanoscale Research Letters 2013 8:66 doi:10.1186/1556-276X-8-66