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Protein detection through different platforms of immuno-loop-mediated isothermal amplification

Mohammad Pourhassan-Moghaddam23, Mohammad Rahmati-Yamchi1*, Abolfazl Akbarzadeh4*, Hadis Daraee23, Kazem Nejati-Koshki2, Younes Hanifehpour5 and Sang Woo Joo5*

Author Affiliations

1 Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 51656, Iran

2 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz 51656, Iran

3 Ian Wark Research Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia

4 Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz 51656, Iran

5 School of Mechanical Engineering, WCU Nanoresearch Center, Yeungnam University, Gyeongsan 712-749, South Korea

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Nanoscale Research Letters 2013, 8:485  doi:10.1186/1556-276X-8-485

Published: 18 November 2013

Abstract

Different immunoassay-based methods have been devised to detect protein targets. These methods have some challenges that make them inefficient for assaying ultra-low-amounted proteins. ELISA, iPCR, iRCA, and iNASBA are the common immunoassay-based methods of protein detection, each of which has specific and common technical challenges making it necessary to introduce a novel method in order to avoid their problems for detection of target proteins. Here we propose a new method nominated as ‘immuno-loop-mediated isothermal amplification’ or ‘iLAMP’. This new method is free from the problems of the previous methods and has significant advantages over them. In this paper we also offer various configurations in order to improve the applicability of this method in real-world sample analyses. Important potential applications of this method are stated as well.

Keywords:
iPCR; iRCA; iNASBA; ELISA; iLAMP; Protein detection