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Nanohybrid structure analysis and biomolecule release behavior of polysaccharide-CDHA drug carriers

Li-Ying Huang1, Ting-Yu Liu2*, Tse-Ying Liu3, Andreas Mevold1, Andri Hardiansyah1, Hung-Chou Liao4, Chin-Ching Lin4 and Ming-Chien Yang1*

Author Affiliations

1 Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan

2 Department of Materials Engineering, Ming Chi University of Technology, New Taipei City 24301, Taiwan

3 Institute of Biomedical Engineering, National Yang-Ming University, 155, Sec. 2, Lih-Nong St, Taipei 112, Taiwan

4 Material and Chemical Research Laboratories, Industrial Technology Research Institute (ITRI), Hsinchu 31040, Taiwan

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Nanoscale Research Letters 2013, 8:417  doi:10.1186/1556-276X-8-417

Published: 8 October 2013


Nanoscaled polymer composites were prepared from polysaccharide chitosan (CS) and Ca-deficient hydroxyapatite (CDHA). CS-CDHA nanocomposites were synthesized by in situ precipitation at pH 9, and the CS-CDHA carriers were then fabricated by ionic cross-linking methods using tripolyphosphate and chemical cross-linking methods by glutaraldehyde and genipin. Certain biomolecules such as vitamin B12, cytochrome c, and bovine serum albumin were loaded into the CS-CDHA carriers, and their release behaviors were investigated. Furthermore, these CS-CDHA carriers were examined by transmission electron microscopy, electron spectroscopy for chemical analysis, and X-ray diffraction. The release behavior of the biomolecules was controlled by the CS/CDHA ratios and cross-linked agents. By increasing the concentration of CS and the concentration of the cross-linking agents, cross-linking within carriers increases, and the release rate of the biomolecules is decreased. Moreover, the release rate of the biomolecules from the CS-CDHA carriers at pH 4 was higher than that at pH 10, displaying a pH-sensitive behavior. Therefore, these CS-CDHA hydrogel beads may be useful for intelligent drug release and accelerate bone reconstruction.

Chitosan; Ca-deficient hydroxyapatite (CDHA); Drug release; Nanohybrids