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Anti-CEA-functionalized superparamagnetic iron oxide nanoparticles for examining colorectal tumors in vivo

Kai-Wen Huang12, Jen-Jie Chieh3*, In-Tsang Lin45, Herng-Er Horng3*, Hong-Chang Yang6 and Chin-Yih Hong7

Author affiliations

1 Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital Taipei, 100, Taiwan

2 Graduate Institute of Clinical Medicine, National Taiwan University, Taipei 100, Taiwan

3 Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan

4 Center for Molecular Imaging and Translational Medicine, Xiamen University, Xiamen 361, China

5 Graduate Institute of Electronics Engineering, National Taiwan University, Taipei 106, Taiwan

6 Department of Electro-Optical Engineering, Kun Shan University, Tainan 710, Taiwan

7 Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan

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Citation and License

Nanoscale Research Letters 2013, 8:413  doi:10.1186/1556-276X-8-413

Published: 8 October 2013

Abstract

Although the biomarker carcinoembryonic antigen (CEA) is expressed in colorectal tumors, the utility of an anti-CEA-functionalized image medium is powerful for in vivo positioning of colorectal tumors. With a risk of superparamagnetic iron oxide nanoparticles (SPIONPs) that is lower for animals than other material carriers, anti-CEA-functionalized SPIONPs were synthesized in this study for labeling colorectal tumors by conducting different preoperatively and intraoperatively in vivo examinations. In magnetic resonance imaging (MRI), the image variation of colorectal tumors reached the maximum at approximately 24 h. However, because MRI requires a nonmetal environment, it was limited to preoperative imaging. With the potentiality of in vivo screening and intraoperative positioning during surgery, the scanning superconducting-quantum-interference-device biosusceptometry (SSB) was adopted, showing the favorable agreement of time-varied intensity with MRI. Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs. This indicates that developed anti-CEA SPIONPs owe the utilities as an image medium of these in vivo methodologies.

Keywords:
Carcinoembryonic antigen; Magnetic nanoparticles; Scanning superconducting-quantum-interference-device biosusceptometry; Magnetic resonance imaging