Effect of particle size of platinum on kidney injury. (A) snPt1 or snPt8 was injected intravenously into mice at the indicated doses. Blood was recovered at 24 h after injection. Serum BUN levels were measured. Data are mean ± SEM (n = 5). Double asterisk (**) connotes significant difference between the snPt1- and snPt8-treated groups (P < 0.01). (B) Histological analysis of kidney tissues in acute snPt1- or snPt8-treated mice. Vehicle or test article (snPt1 or snPt8 at 20 mg/kg) was administered intravenously to mice as a single dose. At 24 h after administration, the kidneys were collected and fixed with 4% paraformaldehyde. Tissue sections were stained with hematoxylin and eosin and observed under a microscope. (C) Acute kidney injury score in mice treated with vehicle, snPt1, or snPt8. Grade 0: none, 1: slight, 2: mild, 3: moderate, 4: severe.
Yamagishi et al. Nanoscale Research Letters 2013 8:395 doi:10.1186/1556-276X-8-395