A comparative in vitro evaluation of self-assembled PTX-PLA and PTX-MPEG-PLA nanoparticles
- Equal contributors
1 Department of Biomaterials, Research Institute for Biomimetics and Soft Matter, College of Materials, Xiamen University, Xiamen 361005, China
2 Department of Materials Science and Engineering, Research Institute for Biomimetics and Soft Matter, College of Materials, Xiamen University, Xiamen 361005, China
3 College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China
4 The First Affiliated Hospital of Xiamen University, Xiamen 361005, China
Nanoscale Research Letters 2013, 8:301 doi:10.1186/1556-276X-8-301Published: 27 June 2013
We present a dialysis technique to direct the self-assembly of paclitaxel (PTX)-loaded nanoparticles (NPs) using methoxypolyethylene glycol-poly(D,L-lactide) (MPEG-PLA) and PLA, respectively. The composition, morphology, particle size and zeta potential, drug loading content, and drug encapsulation efficiency of both PTX-PLA NPs and PTX-MPEG-PLA NPs were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering, electrophoretic light scattering, and high-performance liquid chromatography. The passive targeting effect and in vitro cell viability of the PTX-MPEG-PLA NPs on HeLa cells were demonstrated by comparative cellular uptake and MTT assay of the PTX-PLA NPs. The results showed that the PTX-MPEG-PLA NPs and PTX-PLA NPs presented a hydrodynamic particle size of 179.5 and 441.9 nm, with a polydispersity index of 0.172 and 0.189, a zeta potential of −24.3 and −42.0 mV, drug encapsulation efficiency of 18.3% and 20.0%, and drug-loaded content of 1.83% and 2.00%, respectively. The PTX-MPEG-PLA NPs presented faster release rate with minor initial burst compared to the PTX-PLA NPs. The PTX-MPEG-PLA NPs presented superior cell cytotoxicity and excellent cellular uptake compared to the PTX-PLA NPs. These results suggested that the PTX-MPEG-PLA NPs presented more desirable characteristics for sustained drug delivery compared to PTX-PLA NPs.