Relative GAPDH mRNA expression of HeLa-S3 cells transfected with PEI-NH-CNT/siGAPDH complexes. PEI-NH-SWNTs or PEI-NH-MWNTs were complexed with siGAPDH at mass ratios of 1:1, 10:1, and 20:1 and incubated with HeLa-S3 cells to achieve a final siGAPDH concentration of 30 nM. After 48 h, the mRNA level of GAPDH was analyzed by quantitative PCR. The level of GAPDH gene suppression was quantitated to evaluate the transfection efficiency of PEI-NH-SWNTs and PEI-NH-MWNTs. Control, HeLa-S3 cells cultured in growth medium for 48 h; DharmaFECT, HeLa-S3 cells transfected with siGAPDH using DharmaFECT as transfection reagent. Error bars represent standard deviations (n ≥ 3). *p < 0.05 and **p < 0.01 compared to the control; ##p < 0.01 compared to DharmaFECT.
Huang et al. Nanoscale Research Letters 2013 8:267 doi:10.1186/1556-276X-8-267