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Open Access Nano Express

Voltage-driven translocation behaviors of IgG molecule through nanopore arrays

Lei Liu*, Bing Wang, Jingjie Sha, Yue Yang, Yaozong Hou, Zhonghua Ni and Yunfei Chen

Author Affiliations

Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanics, Southeast University, Nanjing 210096, People's Republic of China

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Nanoscale Research Letters 2013, 8:229  doi:10.1186/1556-276X-8-229

Published: 15 May 2013

Abstract

Nanopore-based biosensing has attracted more and more interests in the past years, which is also regarded as an emerging field with major impact on bio-analysis and fundamental understanding of nanoscale interactions down to single-molecule level. In this work, the voltage-driven translocation properties of goat antibody to human immunoglobulin G (IgG) are investigated using nanopore arrays in polycarbonate membranes. Obviously, the background ionic currents are modulated by IgG molecules for their physical place-holding effect. However, the detected ionic currents do ‘not’ continuously decrease as conceived; the currents first decrease, then increase, and finally stabilize with increasing IgG concentration. To understand this phenomenon, a simplified model is suggested, and the calculated results contribute to the understanding of the abnormal phenomenon in the actual ionic current changing tendency.

Keywords:
Nanopore arrays; Biosensing; Simulation