CAM assay for determining total area of vessels with CellSens Dimension Desktop version 1.3. (A) CAM square area of dimensions 2.5 × 2.5 mm and (B) image with a strengthened local contrast of images by increasing intensity and brightness. (C) For total area calculation, the threshold was set at intensity volumes between 0 and 256 for the shades of red, 0 and 256 for green, 0 and 145 and for blue.
Wierzbicki et al. Nanoscale Research Letters 2013 8:195 doi:10.1186/1556-276X-8-195