Figure 6.

Fluorescence and confocal laser scanning microscopy images of HeLa cells after incubation. (A to C) The fluorescence microscopy images of HeLa cells after incubation with pIRES2-EGFP-loaded and pDsRED-loaded TPGS-b-(PCL-ran-PGA)/PEI nanoparticles. (D to F) Confocal laser scanning microscopy images of HeLa cells after incubation with pIRES2-EGFP-loaded TPGS-b-(PCL-ran-PGA)/PEI nanoparticles at 37.0°C. The cells were stained by DAPI (blue), and the pIRES2-EGFP-loaded TPGS-b-(PCL-ran-PGA)/PEI nanoparticles are in green. The cellular uptake was visualized by overlaying images obtained using DAPI filter and FITC filter: (D) from DAPI channel, (E) from FITC channel, (F) from combined DAPI channel and FITC channel.

Zheng et al. Nanoscale Research Letters 2013 8:161   doi:10.1186/1556-276X-8-161
Download authors' original image