Open Access Nano Express

Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells

Yan Wang, Haixin Cui*, Changjiao Sun, Wei Du, Jinhui Cui and Xiang Zhao

Author Affiliations

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

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Nanoscale Research Letters 2013, 8:127  doi:10.1186/1556-276X-8-127

Published: 15 March 2013

Abstract

We evaluated the performance of green fluorescent magnetic Fe3O4 nanoparticles (NPs) as gene carrier and location in pig kidney cells. When the mass ratio of NPs to green fluorescent protein plasmid DNA reached 1:16 or above, DNA molecules can be combined completely with NPs, which indicates that the NPs have good ability to bind negative DNA. Atomic force microscopy (AFM) experiments were carried out to investigate the binding mechanism between NPs and DNA. AFM images show that individual DNA strands come off of larger pieces of netlike agglomerations and several spherical nanoparticles are attached to each individual DNA strand and interact with each other. The pig kidney cells were labelled with membrane-specific red fluorescent dye 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate and nucleus-specific blue fluorescent dye 4,6-diamidino-2-phenylindole dihydrochloride. We found that green fluorescent nanoparticles can past the cell membrane and spread throughout the interior of the cell. The NPs seem to locate more frequently in the cytoplasm than in the nucleus.

Keywords:
magnetic; fluorescent nanoparticles; gene carrier; location; 75.50.-y; 81.07.-b; 87.85.Rs