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Immobilization of pamidronic acids on the nanotube surface of titanium discs and their interaction with bone cells

Tae-Hyung Koo1, Jyoti S Borah1, Zhi-Cai Xing1, Sung-Mo Moon2, Yongsoo Jeong2 and Inn-Kyu Kang1*

Author Affiliations

1 Department of Polymer Science and Engineering, Kyungpook National University, Daegu, 702-701, South Korea

2 Department of Surface Technology, Korea Institute of Material Science, Changwon-si, 642-831, South Korea

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Nanoscale Research Letters 2013, 8:124  doi:10.1186/1556-276X-8-124

Published: 12 March 2013


Self-assembled layers of vertically aligned titanium nanotubes were fabricated on a Ti disc by anodization. Pamidronic acids (PDAs) were then immobilized on the nanotube surface to improve osseointegration. Wide-angle X-ray diffraction, X-ray photoelectron microscopy, and scanning electron microscopy were employed to characterize the structure and morphology of the PDA-immobilized TiO2 nanotubes. The in vitro behavior of osteoblast and osteoclast cells cultured on an unmodified and surface-modified Ti disc was examined in terms of cell adhesion, proliferation, and differentiation. Osteoblast adhesion, proliferation, and differentiation were improved substantially by the topography of the TiO2 nanotubes, producing an interlocked cell structure. PDA immobilized on the TiO2 nanotube surface suppressed the viability of the osteoclasts and reduced their bone resorption activity.

Pamidronic acid; TiO2 nanotubes; Immobilization; Surface modification; Bone cell