Quantitative analysis of FITC. (a) Thirteen HeLa cells placed on the p-FET sensing area in the middle of the PDMS circular well were visualized with FITC bound to the Aβ peptides expressed on the cell membranes. (b) The photocurrents generated by the fluorescence emitted from the FITC-conjugated Aβ peptides were measured. (c) The number of photons of the single cell in the yellow dotted circle was measured using a PMT. (d) Calibration data of the photon counting as a linear function of the FITC volume.
Kim et al. Nanoscale Research Letters 2012 7:72 doi:10.1186/1556-276X-7-72