Maximum intensity projection of Z-stack, fluorescence confocal image, various fluorescence spectra, and tri-exponential fitting. (a) Maximum intensity projection of Z-stack for section thickness of 50 μm; 500- to 550-nm single-band band-pass filter. (b) Fluorescence confocal image of T3Pyr-functionalized nanowires for excitation at 405 nm. (c) Fluorescence spectrum of the five different areas evidenced in (b), obtained with a Nikon A1 spectral module using a 10-nm resolution. (d, e) Fluorescence spectrum of T3Pyr in solution. (f) Tri-exponential fitting of the decay calculated for an area of interest in (a), yielding lifetimes of 0.3, 1.0, and 2.3 ns.
Fabbri et al. Nanoscale Research Letters 2012 7:680 doi:10.1186/1556-276X-7-680