Confocal laser scanning microscopy of HeLa cells. This is after incubation with loaded-coumarin-6-TPGS-b-(PCL-ran-PGA)/PEI-pDSRED NPs at 37°C. The cells were stained by DAPI (blue), and the plasmids pDsRED-loaded-TPGS-b-(PCL-ran-PGA)/PEI NPs are red. The cellular uptake was visualized by overlaying images obtained. (A) Coumarin-6, (B) RED, (C) DAPI, and (D) co-merge.
Qiu et al. Nanoscale Research Letters 2012 7:666 doi:10.1186/1556-276X-7-666