Figure 3.

Laser-scanned fluorescence images of A549 cells on three different substrates after 21 h of incubation at 37°C. First and second columns show low magnification of actin (phalloidin-stained 532 nm) and nucleus (DRAQ-5-stained 635 nm) of immobilized A549 cells on the three different substrates, respectively. Third column shows the overlapped images of immobilized A549 cells on STR-functionalized nanostructured substrates as well as planar glass substrate (as a control). Fourth column shows the enlarged images of immobilized A549 cells on marked areas A, B, and C in the third column, respectively.