SpringerOpen Newsletter

Receive periodic news and updates relating to SpringerOpen.

Open Access Nano Express

In vitro evaluation of anti-pathogenic surface coating nanofluid, obtained by combining Fe3O4/C12 nanostructures and 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides

Ion Anghel1, Carmen Limban2, Alexandru Mihai Grumezescu3*, Alina Georgiana Anghel4, Coralia Bleotu5 and Mariana Carmen Chifiriuc6

Author Affiliations

1 ENT (Otolaryngology), Carol Davila University of Medicine and Pharmacy, Bucharest, 50474, Romania

2 Department of Pharmaceutical Chemistry, Carol Davila University of Medicine and Pharmacy, Bucharest, 020956, Romania

3 Department of Science and Engineering of Oxidic Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Bucharest, 011061, Romania

4 ENT Clinic, Coltea Hospital, Carol Davila University of Medicine and Pharmacy, Bucharest, 030171, Romania

5 Stefan Nicolau Institute of Virology, Bucharest, 030304, Romania

6 Department of Microbiology, Faculty of Biology, University of Bucharest, Bucharest, 060101, Romania

For all author emails, please log on.

Nanoscale Research Letters 2012, 7:513  doi:10.1186/1556-276X-7-513

The electronic version of this article is the complete one and can be found online at: http://www.nanoscalereslett.com/content/7/1/513


Received:9 August 2012
Accepted:10 September 2012
Published:19 September 2012

© 2012 Anghel et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this paper, we report the design of a new nanofluid for anti-pathogenic surface coating. For this purpose, new 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides were synthesized and used as an adsorption shell for Fe3O4/C12 core/shell nanosized material. The functionalized specimens were tested by in vitro assays for their anti-biofilm properties and biocompatibility. The optimized catheter sections showed an improved resistance to Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 in vitro biofilm development, as demonstrated by the viable cell counts of biofilm-embedded bacterial cells and by scanning electron microscopy examination of the colonized surfaces. The nanofluid proved to be not cytotoxic and did not influence the eukaryotic cell cycle. These results could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with improved anti-biofilm properties.

Keywords:
lauric acid; benzamides; thiourea derivatives; magnetite; anti-biofilm; core/shell nanostructure

Background

In the recent years, the emergence of resistance and multiresistance to antimicrobial substances has led to increasing concerns and interests in finding new antimicrobial agents and identifying new strategies for the treatment of infectious diseases [1-4]. Thiourea derivatives possess many biological activities, including antimicrobial activity, having interesting applications in numerous fields (in agriculture, as ligands useful in coordination chemistry, in analytical chemistry, in anion recognition, and in catalysis).

The synthesis and antibacterial activity of thiourea derivatives has been the subject of numerous investigations. Some new substituted 1,3,5-triazine with 1,2,4-triazole and substituted thiourea and urea were previously synthesized and evaluated for their in vitro inhibitory activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, some of them showing excellent antimicrobial activity [5].

Sarmah and coworkers synthesized and characterized new compounds using the substitution of chlorine in cyanuric chloride by some moieties with biological importance, such as substituted thiourea and heterocyclic systems, in order to achieve enhanced antimicrobial activity against different bacterial and fungal strains, favored by the presence of electron-withdrawing groups on the aromatic ring as compared to compounds with electron-donating groups [6]. The (E)-N-[4-(benzamidomethyleneamino)phenylcarbamothioyl]benzamide synthesized by Kurt et al. exhibited in vitro antibacterial activity against B. subtilis[7].

The biological significance of thioureas and 2-aminobenzothiazoles stimulated research to investigate the synergistic effects of these moieties to afford the design of a new class of heterocyclic thioureas. The new 1-aroyl-3-(substituted-2-benzothiazolyl)-thioureas were found to exhibit moderate to potent activity towards S. aureus, B subtilis, P. aeruginosa, and E. coli, as compared to the standard drugs [8]. Some compounds of the novel series of benzothiazolyl thiourea derivatives were equipotent with ampicillin against S. aureus and E. coli and showed good activity against Mycobacterium tuberculosis H37Rv. Also, they were evaluated for in vitro cytotoxicity against MCF-7 breast cancer cells [9].

N-phenyl- and N-benzoylthiourea derivatives obtained by a simple and inexpensive manner display selective antimicrobial activities against Cladosporium cladosporioides, B. subtilis, and Micrococcus luteus. Benzoylthioureas were more active than the corresponding phenyl ones [10].

5-Thiourea oxazolidinones were synthesized and their antibacterial activity against Gram-positive bacteria including methicillin-resistant S. aureus and vancomycin-resistant Enterococcus was evaluated. This activity was significantly affected by the compounds' lipophilicity, especially the calculated log p value [11].

Medical device-related infections account for a substantial morbidity, causing an important economic burden by the increase of antibiotic treatment and hospitalization days, as well as the health-care-associated costs [12-14]. The topological and chemical characteristics of the medical device surface are influencing microbial adherence, the less likely to be colonized being the perfectly smooth, hydrophilic ones. A lot of strategies have been employed to prevent medical device-related infections, one of them being surface modification to prevent microbial population and biofilm formation by (1) the chemical modification of the surface with protein, (2) the modification of the surface with quaternary ammonium salts acting as bacteria-repellent coatings, (3) the incorporation and release of antibiotics from the surface, and (4) the use of noble metals and especially silver on the surface as antimicrobial coatings [15].

There are a lot of reports on the antimicrobial and anti-biofilm properties of different types of nanoparticles, especially metals or metallic oxide-containing ones (silver, copper, gold, and ZnO) [16-26], as well as core/shell nanosystems (e.g., CoFe2O4/oleic acid, Fe3O4/oleic acid, and Fe3O4/PEG600) [27-31] that could be manipulated and improved, potentially providing a new method for treating antibiotic-resistant device-related infections [32-35]. In the last year, two articles were published opening a new perspective for obtaining new antimicrobial and anti-biofilm surfaces, based on hybrid functionalized nanostructured biomaterials [36,37]. The first one showed that the Rosmarinus officinalis essential oil-coated Fe3O4/C18 strongly inhibited the adherence ability and biofilm development of the C. albicans and Candida tropicalis tested strains to the catheter surface, and the second showed that the usnic acid-coated Fe3O4/C18 strongly inhibited the adherence ability and biofilm development of the S. aureus tested strain to the coverslip surface, as shown by viable cell counts (VCCs) and confocal laser scanning microscopy. These material-based approaches to the control of fungal/microbial adherence could provide both (1) new tools to study mechanisms of fungal/microbial virulence and biofilm formation and (2) approaches to the design of film-coated surfaces or to treat the surfaces of solid and fiber-based materials that prevent or disrupt the formation of fungal/microbial biofilms.

Taking into consideration the aforementioned significant antimicrobial activity of thioureas, in order to continue our work on the evaluation of the bioactivity of compounds with thiourea moiety, we decided to design a new nanosystem combining new 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides and a Fe3O4/C12 core/shell nanostructure with up to 5-nm size for catheter surface coating, with an improved resistance to S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 colonization and subsequent in vitro biofilm development.

Methods

Fe3O4/C12: synthesis, characterization, and biological assay

In a previous study [38], we reported the successful synthesis of magnetite coated with lauric acid (C12) and its characterization by X-ray diffraction (XRD), transmission electron microscopy (TEM), thermogravimetric analysis, and Fourier transform infrared spectroscopy (FT-IR). The obtained nanopowder was identified as magnetite by XRD. The dimension of the core/shell structure not exceeding 5 nm and its spherical shape were confirmed by TEM analysis. The FT-IR analysis identified the lauric acid on the surface of the magnetite nanoparticles. Treatment for 24 h with Fe3O4/C12 is not cytotoxic on the HEp-2 cell line, this aspect representing an advantage for the in vivo use of these nanostructures [38]. Briefly, lauric acid was solubilized in a known volume of distilled-deionized water, corresponding to a 1% (w/w) solution, under stirring at room temperature. Then, a basic aqueous solution consisting of 28% NH3 was added to the lauric acid solution. Thereafter, Fe2+ and Fe3+ (1:2 molar ratio) were dropped under permanent stirring, leading to the formation of a black precipitate. The core/shell was washed with methanol and separated with a strong NdFeB permanent magnet.

2-((4-Ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides: general synthesis and characterization

2-((4-Ethylphenoxy)methyl)-N-(phenylcarbamothioyl)-benzamide (1a) and 2-((4-ethylphenoxy)methyl)-N-(2-chlorophenylcarbamothioyl)-benzamide (1b) were prepared according to procedures described by Limban et al. [39] and according to the scheme plotted in Figure 1.

thumbnailFigure 1. The pathway for the synthesis of the new thioureides (1a, 1b).

The new thioureides (1a, 1b) were prepared by heating 2-((4-ethylphenoxy)methyl)benzoyl isothiocyanate (2) (obtained from the corresponding chloride (3)) with primary aromatic amines in dry acetone under reflux. The acylthioureas are white crystalline solids, insoluble in water, and soluble in acetone and chloroform at normal temperature and in short-chain aliphatic alcohols, benzene, toluene, and xylene at higher temperatures. Their structures were confirmed by elemental analyses, IR, and NMR spectral data.

Fabrication of coating nanofluid

The adsorption shell represented by 15 mg of (1a, 1b) was solubilized in 1 mL of chloroform together with 135 mg Fe3O4/C12 nanopowder. This mix was grounded until complete evaporation of chloroform. This step was repeated thrice in order to obtain a uniform distribution of the organic compounds on the surface of the spherical nanostructure. The fabrication was performed by coating a 1-cm catheter section (the prosthetic device was obtained from a local provider of ENT Coltea Bucharest Hospital) with the nanofluid represented by a suspended core/shell/adsorption shell in CHCl3 (0.33% w/v). The layer of the nanostructured coating on the catheter sections was achieved by submerging the catheter pieces in 5 mL of nanofluid aligned in a magnetic field of 100 kgf applied for 1 s, followed by extemporaneous drying at room temperature. The rapid drying was facilitated by the convenient volatility of chloroform. The coated catheter sections were then sterilized by ultraviolet irradiation for 15 min. Figure 2 presents the schematic illustration of biofilm development in the presence/absence of the coating nanofluid.

thumbnailFigure 2. Schematic illustration of biofilm development in the presence/absence of the coating nanofluid.

In vitro microbial biofilm development to the catheter sections with modified surface

The biofilms were developed using S. aureus ATCC 25923 and P. aeruginosa ATTC 27853 strains. The microbial adherence ability was investigated in six multiwell plates, in which there have been placed 1-cm catheter pieces with and without nanostructured coating. Plastic wells were filled with a liquid medium, inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 72 h at 30°C. After 24 h, the culture medium was removed, the catheters were washed three times with phosphate-buffered saline (PBS) in order to remove the non-adherent strains, and fresh glucose broth was added. Also, VCCs have been achieved for both working variants (coated and uncoated catheter pieces) at 24, 48, and 72 h in order to assess the biofilm-forming ability of the two strains. The adhered cells have been removed from the catheter sections by vortexing and brief sonication, and serial dilutions ranging from 10−1 to 10−4 of the obtained inocula have been spotted on Muller-Hinton agar, incubated for 24 h at 30°C and assessed for VCCs.

Cell cycle analysis

Plastic slides uncoated or coated with the obtained nanofluid were put into wells of 3.5-cm diameter, and thereafter, 3.5 × 105 HCT8 cells and HEp-2 cells were seeded onto the slides and maintained for 24 h at 37°C in 5% CO2 and humid condition. The morphology of cells grown on the plastic or coated slides was checked using an inverted microscope after 24 h. For cell cycle analysis, cells were harvested, washed with PBS (pH 7.5), fixed in 70% cold ethanol, and maintained at −20°C overnight. Each sample was washed with PBS, treated with 100 μg/mL RNase A for 15 min, and colored with 10 μg/mL propidium iodide by incubation at 37°C for 1 h. After cell staining with propidium iodide, the acquisition was done using a Coulter Epics XL Flowcytometer (Fullerton, CA, USA). Data were analyzed using FlowJo software (Tree Star, Inc., Ashland, OR, USA) and expressed as fractions of cells in the different cell cycle phases.

Assessment of cytotoxicity by fluorescent microscopy

HCT8 cells (3.5 × 105) were seeded in each well of a 24-well plate. After 24 h, the cells were treated with 50 μg/mL of the obtained nanofluid. The effect of the nanofluid was evaluated after 24 h by adding 100 μL PI (0.1 mg/mL) and 100 μL fluorescein diacetate. In order to evaluate dead cells (red) and viable ones (green), fluorescence was quantified using a Carl Zeiss Observer.D1 microscope (Oberkochen, Germany).

Statistical analysis

The statistical significance of the obtained results was analyzed using GraphPad Prism version 5.04 for Windows [40]. We used for comparison the number of colony-forming units per milliliter as revealed by the readings of three values/experimental variants. Logarithmated values were used for statistical analysis. We chose to employ two-way ANOVA and Tukey's multiple comparison tests for revealing significant differences among the analyzed groups.

Results and discussion

Catheter-related infections continue to be a significant source of morbidity and mortality in patients requiring catheterization [41] and increase medical expenses by prolonging hospitalization. One of the most common etiologies of catheter infections are staphylococci, either coagulase-negative staphylococci or S. aureus, and P. aeruginosa. There are a lot of studies trying to demonstrate the efficiency of different substances as anti-biofilm-coated agents in reducing the incidence of catheter-associated biofilm infections (i.e., cefazolin, teicoplanin, vancomycin, silver sulfadiazine, chlorhexidine-silver sulfadiazine, minocycline-rifampin, lysostaphin, ciprofloxacin, and protamine sulfate combinations). There are a lot of studies reporting the efficacy of antibiotic-bonded catheters in preventing microbial biofilms from developing. It was demonstrated that the immersion of central venous catheters and arterial catheters in a 50 mg/mL cefazolin solution reduced the catheter colonization with Staphylococcus epidermidis from 40% to 2%, proving that antibiotic bonding is an efficient, safe, and cost-effective method of reducing intravascular catheter infections in patients who are in intensive care units [42,43]. Also, other research teams demonstrated that catheter coating with lysostaphin might be more suitable than antimicrobial bonding, due to the rapid coating time of catheters with minimal on-site catheter preparation, and the rapidity of kill would eradicate adherent bacteria within a very short amount of time, eliminating the risk of infections [44]. Nano-silver coatings have been applied to several medical devices, of which catheters, drains, and wound dressings are the most prominent [15].

Previous studies have demonstrated that the synergism between ciprofloxacin and protamine sulfate significantly enhanced the efficacy of ciprofloxacin against planktonic and biofilm-grown P. aeruginosa cells [45]. In our study, concerning P. aeruginosa biofilms, catheter coating by nanoparticles alone proved to be significantly more prone to bacterial colonization (p < 0.0001) at 24 h than the standard catheter and the catheter sections either immersed in the antimicrobial solution or coated with the nanoparticles loaded with the newly synthesized compounds (Figure 3). The compounds incorporated in nanoparticles (1a) and (1b) proved to be more efficient than the nanoparticles alone against P. aeruginosa biofilm development at 24 h (p < 0.0001). It is to be noticed that at 72 h, the compounds incorporated in nanoparticles (1a) exhibited a very significant improvement of the anti-biofilm activity as compared with the catheter sections immersed in the soluble compound (1a) (p < 0.0001).

thumbnailFigure 3. The VVCs of P. aeruginosa cells embedded in biofilms developed on different catheter sections. C, uncoated catheter; C + N, catheter coated with nanoparticles; 1a, 1b, catheters immersed in the compound solution; 1a + N, 1b + N, catheters coated with N and the chemical compound solution.

Concerning S. aureus biofilms, at 24 h, as for the case of P. aeruginosa biofilms, the catheters coated only with nanoparticles were significantly more colonized as compared with the uncoated catheter (p < 0.0001; Figure 4). However, compound (1a) exhibited a strong anti-biofilm activity, the results being very significant when comparing either the uncoated catheter versus the catheter immersed in the soluble compound (p < 0.0001) or the catheter coated with nanoparticles versus the catheter coated with nanoparticles and compound (1a) (p < 0.0001).

thumbnailFigure 4. The VVCs of S. aureus cells embedded in biofilms developed on different catheter sections. C, uncoated catheter; C + N, catheter coated with nanoparticles; 1a, 1b, catheters immersed in the compound solution; 1a + N, 1b + N, catheters coated with N and the chemical compound solution.

At 48 h, both compounds (1a) and (1b) in soluble form exhibited protective activity against S. aureus mature biofilm development (p < 0.0001). Only compound (1b) significantly improved the anti-biofilm of the catheter surface in the presence of nanoparticles (p < 0.0001). Very statistically significant results have been obtained at 72 h when a strong inhibitory effect of S. aureus biofilm development was obtained for the catheter coating constituted of nanoparticles loaded with compounds (1a) and (1b), as compared with the results obtained for the catheter sections immersed in the compound solution alone or coated only with nanoparticles (p < 0.0001).

Our results are demonstrating that the nanoparticle layer alone is not protective against microbial colonization, probably affecting the roughness and the electric charge of the catheter surface, favoring the interaction with the microbial surfaces. On the other hand, our study is clearly proving that the nanoparticle layer is interacting differently with the incorporated substances, influencing their release time in active forms and their antimicrobial activity.

The coating system represented by nanoparticles loaded with (1a) proved to be efficient in preventing both the initial formation as well the development of mature microbial biofilms formed by S. aureus and P. aeruginosa, demonstrating the efficiency of the nanoparticle coating in the delivery of the chemical compound in active forms for a long period of time. These results are also proving that the obtained nanostructured coating agent is not only preventing bacteria to adhere to the catheter surface, but also acting as a biofilm dispersal agent. The molecular mechanisms of bacterial biofilm dispersal are only beginning to be elucidated; however, biofilm dispersal is a promising area of research that may lead to the development of novel agents that inhibit biofilm formation or promote biofilm cell detachment. Such agents may be useful for the prevention and treatment of biofilms in a variety of industrial and clinical settings [46].

Scanning electron microscopy (SEM) was used for the evaluation of catheter surface, detection of biofilm, and studying the effect of the coating agents on biofilm development. This technique provides excellent visualization of glycocalyx, which is one of the most prominent features of biofilms and a crucial research subject in searching for alternative antimicrobial and anti-adherent agent treatment [47]. After 24, 48, and 72 h of incubation, the samples were removed from the plastic wells, washed three times with PBS, fixed with cold methanol, and dried before microscopic examination. The samples were visualized using a HITACHI S2600N electron microscope (Chiyoda-ku, Japan), at 25 keV, in primary electron fascicle, on samples covered with a thin silver layer. The culture-based findings were substantiated by the SEM studies of colonized catheter samples, showing the gradual decrease of microbial colonization from the uncoated catheter to the catheter pieces immersed in the compound solution to the catheter pelliculized with nanofluids (nanoparticles and compound solution; Figures 5, 6, and 7).

thumbnailFigure 5. SEM images showing the uncoated catheter surface colonized with S. aureus harvested at 48 h. The images show a mature biofilm with a rich matrix (left, ×2,500; right, ×5,000).

thumbnailFigure 6. SEM images showing compound (1b)-coated catheter surface colonized with S. aureus harvested at 48 h. The images show a dense biofilm (left, ×2,500; right, ×10,000).

thumbnailFigure 7. SEM images showing nanofluid-coated catheter surface colonized with S. aureus harvested at 48 h. The images show a reduced biofilm with rare bacterial cells (left, ×1,500; right, ×10,000).

When HEp-2 cells were grown on slides coated with the obtained nanofluid, containing nanoparticles and compounds (1a) and (1b), no changes were observed in their morphology (Figure 8). The analysis of the cell cycle of HEp-2 cells grown on slides coated with nanofluid showed no significant changes of cell cycle phases (Figure 9). Only unsignificant cell death rate was induced by a concentration of 50 μg/mL (Figure 10).

thumbnailFigure 8. Inverted microscope images of HEp-2 cells grown on slides treated with the obtained nanofluid. Phase contrast microscopy, ×200; left, (1a); right, (1b).

thumbnailFigure 9. HCT8 cell cycle analysis after 24-h development on microscopic slides coated with the obtained nanofluid. From left to right: (1a), (1b), and control.

thumbnailFigure 10. The effects of the obtained nanofluids on HCT8 cell viability. IF, ×200; left, (1a); right, (1b).

Conclusions

Our aim was to combine the unique properties of newly synthesized biocompatible Fe3O4/C12 nanoparticles with 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides in order to obtain functionalized catheter surfaces with improved resistance to in vitro microbial colonization and biofilm development. The obtained nanofluids proved to be not cytotoxic and did not influence the eukaryotic cell cycle. The long-lasting efficacy of compound (1a) loaded on nanoparticles could be regarded as a future solution to provide persistent, broad-spectrum antibacterial effects with minimal side effects. Taken together, our results could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with improved anti-biofilm properties.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

IA and AMG participated in the design and coordination of the study. AMG and MCC conceived the study and drafted the manuscript. CL performed the synthesis and characterization of new thiourea. AMG performed the synthesis and characterization of the nanofluid and coated materials. CB and AGA performed the biological studies. All authors read and approved the final manuscript.

Acknowledgments

This paper is supported by the PN-II-PT-PCCA-2011-3.2-0284: ‘Novel nanostructured prosthetic tubular devices with antibacterial and antibiofilm properties induced by physicochemical and morphological changes’ and Ideas (contract no. 154/2011) funded by the National University Research Council in Romania.

References

  1. Anghel I, Chifiriuc MC, Mitache M, Marutescu L, Anghel AG, Popa M, Pelinescu D, Bleotu C, Lazar V: Phenotypic and genotypic assessment of virulence factors in betahemolytic streptococci isolated from kindergarten infantile population, with or without clinical symptoms, including scarlet fever.

    Farmacia 2012, 60:21-31. OpenURL

  2. Kumar N, Shalini K, Drabu S: Synthesis and pharmacological screening of various new quinazolin-4-one derivatives as anti-inflammatory and antifungal agents.

    Biointerface Res Appl Chem 2011, 1:203-208. OpenURL

  3. Shalini K, Kumar N, Sharma PK: Synthesis of N-((2-substituted phenyl)-4, 5-diphenyl-1 H-imidazol-1yl)(phenyl)methyl) substituted amine derivatives, spectral characterization and their pharmacological evaluation.

    Biointerface Res Appl Chem 2011, 1:184-190. OpenURL

  4. Prasad SK, Kumar SL, Prasad M, Jayalakshmi B, Revanasiddappa HD: Synthesis, spectral characterization, DNA interaction studies, anthelmintic and antimicrobial activity of transition metal complexes with 3-(2-hydroxybenzylideneamino)-2-methylquinazolin-4(3 H)-one and 1,10-phenanthroline.

    Biointerface Res Appl Chem 2011, 1:127-138. OpenURL

  5. Sarmah KN, Sarmah NK, Patel TV, Kurmi KB: Synthesis, characterization, antimicrobial studies of certain triazole containing s-triazine derived compound.

    Int J Comput Eng Res 2012, 2:289-293. OpenURL

  6. Sarmah KN, Patel TV: Synthesis, characterization, antimicrobial studies of certain s-triazine derived compounds and analogues.

    Arch Appl Sci Res 2011, 3:428-436. OpenURL

  7. Kurt G, Sevgi F, Mercimek B: Synthesis, characterization, and antimicrobial activity of new benzoylthiourea ligands.

    Chem Pap 2009, 63:548-553. Publisher Full Text OpenURL

  8. Saeed A, Rafique H, Hameed A, Rasheed S: Synthesis and antibacterial activity of some new 1-aroyl-3-(substituted-2-benzothiazolyl)-thioureas.

    Pharm Chem J 2008, 42:191-195. Publisher Full Text OpenURL

  9. Abdel-Rahman HM, Morsy MA: Novel benzothiazolyl urea and thiourea derivatives with potential cytotoxic and antimicrobial activities.

    J Enzyme Inhib Med Chem 2007, 22:57-64. PubMed Abstract | Publisher Full Text OpenURL

  10. Cunha S, Macedo FC Jr, Costa GAN, Rodrigues MTJ, Verde RBV, de Souza Neta LC, Vencato I, Lariucci C, Sa FP: Antimicrobial activity and structural study of disubstituted thiourea derivatives.

    Monatsh Chem Chem Mon 2007, 138:511-516. OpenURL

  11. Tokuyama R, Takahashi Y, Tomita Y, Tsubouchi M, Yoshida T, Iwasaki N, Kado N, Okezaki E, Nagata O: Structure-activity relationship (SAR) studies on oxazolidinone antibacterial agents. 2. Relationship between lipophilicity and antibacterial activity in 5-thiocarbonyl oxazolidinones.

    Chem Pharm Bull 2001, 49:353-360. PubMed Abstract | Publisher Full Text OpenURL

  12. Hellmann M, Mehta SD, Bishai DM, Mears SC, Zenilman JM: The estimated magnitude and direct hospital costs of prosthetic joint infections in the United States, 1997 to 2004.

    J Athroplasty 2010, 25:766-771. Publisher Full Text OpenURL

  13. Tokarczyk AJ, Greenberg SB, Vender JS: Death, dollars, and diligence: prevention of catheter-related bloodstream infections must persist.

    Crit Care Med 2009, 37:2320-2321. PubMed Abstract | Publisher Full Text OpenURL

  14. Anghel I, Chifiriuc MC, Anghel GA: Pathogenic features and therapeutical implications of biofilm development ability in microbial strains isolated from rhinologic chronic infections.

    Farmacia 2011, 59:770-783. OpenURL

  15. Knetsch MLW, Koole LH: New strategies in the development of antimicrobial coatings: the example of increasing usage of silver and silver nanoparticles.

    Polymers 2011, 3:340-366. Publisher Full Text OpenURL

  16. Jun Sung K, Eunye K, Kyeong Nam Y, Jong-Ho K, Sung Jin P, Hu Jang L, So Hyun K, Young Kyung P, Yong Ho P, Cheol-Yong H, Yong-Kwon K, Yoon-Sik L, Dae Hong J, Myung-Haing C: Antimicrobial effects of silver nanoparticles.

    Nanomedicine: Nanotechnology, Biology, and Medicine 2007, 3:95-101. Publisher Full Text OpenURL

  17. Rai A, Prabhune A, Perry CC: Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings.

    J Mater Chem 2010, 20:6789-679. Publisher Full Text OpenURL

  18. Gajjar P, Pettee B, Britt DW, Huang W, Johnson WP, Anderson AJ: Antimicrobial activities of commercial nanoparticles against an environmental soil microbe, Pseudomonas putida KT2440.

    J Biol Eng 2009, 3:9. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  19. Subhasree RS, Selvakumar D, Kumar NS: Hydrothermal mediated synthesis of ZnO nanorods and their antibacterial properties.

    Lett Appl NanoBioSci 2012, 1:2-7. OpenURL

  20. Andronescu E, Grumezescu AM, Ficai A, Gheorghe I, Chifiriuc M, Mihaiescu DE, Lazar V: In vitro of antibiotic magnetic dextran microspheres complexes against Staphylococcus aureus and Pseudomonas aeruginosa strains.

    Biointerface Res Appl Chem 2012, 2:332-338. OpenURL

  21. Baier J, Strumberger R, Berger F, Atanasova P, Welzel U, Bill J: Mineralization and particle growth kinetics of ZnO in the presence of gelatin.

    Biointerface Res Appl Chem 2012, 2:339-349. OpenURL

  22. Marquez MF, Herrera GM, Campo T, Cotto MC, Duconge J, Sanz JM, Elizalde E, Perales O, Morant C: Preparation of hollow magnetite microspheres and their applications as drugs carriers.

    Nanoscale Res Lett 2012, 7:210. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  23. Labouta IH, Schneider M: Tailor-made biofunctionalized nanoparticles using layer-by-layer technology.

    Int J Pharm 2010, 395:236. PubMed Abstract | Publisher Full Text OpenURL

  24. Mantle MD: Quantitative magnetic resonance micro-imaging methods for pharmaceutical research.

    Int J Pharm 2011, 417:173. PubMed Abstract | Publisher Full Text OpenURL

  25. Karmali RJ, Bartakke A, Borker VP, Rane KS: Bactericidal action of N doped ZnO in sunlight.

    Biointerface Res Appl Chem 2011, 1:57-63. OpenURL

  26. Mihaiescu DE, Horja M, Gheorghe I, Ficai A, Grumezescu AM, Bleotu C, Chifiriuc MC: Water soluble magnetite nanoparticles for antimicrobial drugs delivery.

    Lett Apll NanoBioSci 2012, 1:45-49. OpenURL

  27. Chifiriuc MC, Lazar V, Bleotu C, Calugarescu I, Grumezescu AM, Mihaiescu DE, Mogoşanu DE, Buteica AS, Buteica E: Bacterial adherence to the cellular respectively inert substrate in the presence of magnetic CoFe2O4 and Fe3O4/oleic acid – core/shell nanoparticle.

    Digest J Nanomat Biostr 2011, 6:37-42. OpenURL

  28. Saviuc C, Grumezescu AM, Holban A, Chifiriuc C, Mihaiescu D, Lazar V: Hybrid nanostructurated material for biomedical applications.

    Biointerface Res Appl Chem 2011, 1:64-71. OpenURL

  29. Saviuc C, Grumezescu AM, Holban A, Bleotu C, Chifiriuc C, Balaure P, Lazar V: Phenotypical studies of raw and nanosystem embedded Eugenia carryophyllata buds essential oil antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus strains.

    Biointerface Res Appl Chem 2011, 1:111-118. OpenURL

  30. Wang H, Wang S, Liao Z, Zhao P, Su W, Niu R, Chang J: Folate-targeting magnetic core–shell nanocarriers for selective drug release and imaging.

    Int J Pharm 2011, 430:343. OpenURL

  31. Schweiger C, Pietzonka C, Heverhagen J, Kissel T: Novel magnetic iron oxide nanoparticles coated with poly(ethylene imine)-g-poly(ethylene glycol) for potential biomedical application: synthesis, stability, cytotoxicity and MR imaging.

    Int J Pharm 2011, 408:130-137. PubMed Abstract | Publisher Full Text OpenURL

  32. Chifiriuc MC, Grumezescu AM, Saviuc C, Croitoru C, Mihaiescu DE, Lazar V: Improved antibacterial activity of cephaloporins loaded in magnetic chitosan microspheres.

    Int J Pharm 2012. Publisher Full Text OpenURL

  33. Mihaiescu DE, Grumezescu AM, Balaure PC, Mogosanu DE, Traistaru V: Magnetic scaffold for drug targeting: evaluation of cephalosporins controlled release profile.

    Biointerface Res Appl Chem 2011, 1:191-195. OpenURL

  34. Akbarzadeh A, Samiei M, Davaran S: Magnetic nanoparticles: preparation, physical properties, and applications in biomedicine.

    Nanoscale Res Lett 2012, 7:144. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  35. Medeiros SF, Santos AM, Fessi H, Elaissari A: Stimuli-responsive magnetic particles for biomedical applications.

    Int J Pharm 2011, 403:139. PubMed Abstract | Publisher Full Text OpenURL

  36. Chifiriuc MC, Grumezescu V, Grumezescu AM, Saviuc CM, Lazar V, Andronescu E: Hybrid magnetite nanoparticles/Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity.

    Nanoscale Res Lett 2012, 7:209. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  37. Grumezescu AM, Saviuc C, Chifiriuc MC, Hristu R, Mihaiescu DE, Balaure P, Stanciu G, Lazar V: Inhibitory activity of Fe3O4/oleic acid/usnic acid-core/shell/extra-shell nanofluid on S. aureus biofilm development.

    IEEE T. Nanobiosci 2011, 10:269-274. OpenURL

  38. Grumezescu AM, Andronescu E, Ficai A, Mihaiescu DE, Vasile BS, Bleotu C: Synthesis, characterization and biological evaluation of a magnetite/lauric acid core/shell nanosystem.

    Lett Appl NanoBioSci 2012, 1:31-35. OpenURL

  39. Limban C, Missir AV, Chiriţă IC, Niţulescu GM, Ilie C, Căproiu MT: Some new 2-(4-ethyl-phenoxymethyl)benzoic acid thioureides: synthesis and spectral characterisation.

    Rev Chem (Bucharest) 2009, 60:657-661. OpenURL

  40. GraphPad Software[www.graphpad.com]

  41. O’Grady NP, Alexander M, Dellinger EP, Gerberding JL, Heard SO, Maki DG, Masur H, McCormick RD, Mermel LA, Pearson ML, Raad II, Randolph A, Weinstein RA: Guidelines for the prevention of intravascular catheter-related infections.

    Morb Mortal Wkly Rep Recomm Rep 2002, 51:1-29. OpenURL

  42. Pfaller MA, Rempe LE, Jebson PJR, Kamal D: Reduced intravascular catheter infection by antibiotic bonding a prospective, randomized, controlled trial.

    MBBS, FFARCS, DEAA; FFARCS JAMA 1991, 265:2364-2368. OpenURL

  43. Bassetti S, Hu J, D’Agostino RB, Sherertz RJ: Prolonged antimicrobial activity of a catheter containing chlorhexidine-silver sulfadiazine extends protection against catheter infections in vivo.

    Antimicrob Agents Chemother 2001, 45:1535-1538. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  44. Shah A, Mond J, Walsh S: Lysostaphin-coated catheters eradicate Staphylococccus aureus challenge and block surface colonization.

    Antimicrob Agents Chemother 2004, 48:2704-2707. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  45. Soboh F, Khoury AE, Zamboni AC, Davidson D, Mittelman MW: Effects of ciprofloxacin and protamine sulfate combinations against catheter-associated Pseudomonas aeruginosa biofilms.

    Antimicrob Agents Chemother 1995, 39:1281-1286. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  46. Kaplan JB: Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses.

    JDR 2010, 89(3):205-218. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  47. Abd El-Baky RM: Scanning Electron Microscopy. In Application of scanning electron microscopy for the morphological study of biofilm in medical devices. Edited by Viacheslav K. Rijeka: InTech; 2012. OpenURL