DCN over-expression inhibited expression of TGF-β1 and collagen IV in MC.A, The protein level of DCN was assessed by Western blotting in PEI–pDCN transfected MC. Normal mesangial cells were used as a control. D1–D6 are different cell clones. B, The relative levels of DCN, TGF-β1 and collagen IV protein were determined by Western blotting in D6. Lanes 1, normal mesangial cells; 2, pcDNA3.1A-transfected MC; and 3, DCN-transfected MC (D6). Data are presented as mean ± SD. *P <0.01 vs MC and MC/pcDNA3.1A. The experiment was done at least three times.
Sun et al. Nanoscale Research Letters 2012 7:451 doi:10.1186/1556-276X-7-451