Evaluation of the transfection efficiency in MC with PEI–pEGFP complexes.A, Fluorescence micrograph of MC transfected with PEI–pEGFP complexes for 48 h at different N/P ratios. (a) MC were transfected with Lipofectamine™ 2000 as a positive control. (b) N/P 1, (c) N/P 5, (d) N/P 10, (e) N/P 15 and (f) N/P 20. (×100) B, The transfection efficiency of MC with different N/P ratios was evaluated by flow cytometry. Cells treated with naked plasmid were used as a negative control. C, Cytotoxicity analysis of MC with different N/P ratios by the MTT test. Cells treated with naked plasmid were used as a negative control and cells transfected with Lipofectamine™ 2000 were used as a positive control. *P <0.01 vs control. The results are representative of at least three similar experiments.
Sun et al. Nanoscale Research Letters 2012 7:451 doi:10.1186/1556-276X-7-451