DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers
1 The Inter-Departmental Program of Biotechnology, Technion – Israel Institute of Technology, Haifa, 32000, Israel
2 Department of Biotechnology and Food Engineering, Technion – Israel Institute of Technology, Haifa, 32000, Israel
3 Karlsruhe Institute of Technology, DFG – Center for Functional Nanostructures, Karlsruhe, 76131, Germany
4 Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, 76100, Israel
5 The Russell Berrie Nanotechnology Institute, Technion – Israel Institute of Technology, Haifa, 32000, Israel
Nanoscale Research Letters 2012, 7:443 doi:10.1186/1556-276X-7-443Published: 8 August 2012
Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild dehybridization conditions.