Table 4
Relative gene expression in the heart of chickens treated with different levels of AgNano
Gene symbol	Treatment					ANOVA
	Control	10 ppm AgNano	20 ppm AgNano	10 ppm AgNanoc	20 ppm AgNanoc	SEM	P value
FGF2a	0.97†	0.85	0.79	0.84	0.69‡	0.028	0.0443
FGF2b	1.47	1.32	1.34	1.15	1.31	0.054	0.5839
VEGFAa	0.59	0.68	0.78	0.73	0.61	0.026	0.0888
VEGFAb	0.75†	0.93	1.12‡	0.84	1.14‡	0.044	0.0027

^aNormalised to ACTB; ^bnormalised to EEF1A2; ^ceggs were in ovo injected; ^†,‡different symbols indicate statistically significant differences (P < 0.05). AgNano, nanoparticles of colloidal silver; ANOVA, analysis of variance; SEM, standard error of the mean.

Hotowy et al. Nanoscale Research Letters 2012 7:418   doi:10.1186/1556-276X-7-418