SpringerOpen Newsletter

Receive periodic news and updates relating to SpringerOpen.

Open Access Nano Express

Light-harvesting bio-nanomaterial using porous silicon and photosynthetic reaction center

Kata Hajdu1*, Csilla Gergely2, Marta Martin2, László Zimányi3, Vivechana Agarwal4, Gabriela Palestino5, Klára Hernádi6, Zoltán Németh6 and László Nagy1

Author affiliations

1 Department of Medical Physics and Informatics, University of Szeged, Szeged, H-6720, Hungary

2 Laboratoire Charles Coulomb, UMR 5221 CNRS - Université Montpellier 2, Montpellier, F-34095, France

3 Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, H-6701, Hungary

4 CIICAP - Universidad Autonoma del Estado de Morelos, Col Chamilpa, Cuernavaca, 62209, Mexico

5 Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, 78270, Mexico

6 Department of Applied and Environmental Chemistry, University of Szeged, Szeged, H-6720, Hungary

For all author emails, please log on.

Citation and License

Nanoscale Research Letters 2012, 7:400  doi:10.1186/1556-276X-7-400

Published: 17 July 2012

Abstract

Porous silicon microcavity (PSiMc) structures were used to immobilize the photosynthetic reaction center (RC) purified from the purple bacterium Rhodobacter sphaeroides R-26. Two different binding methods were compared by specular reflectance measurements. Structural characterization of PSiMc was performed by scanning electron microscopy and atomic force microscopy. The activity of the immobilized RC was checked by measuring the visible absorption spectra of the externally added electron donor, mammalian cytochrome c. PSi/RC complex was found to oxidize the cytochrome c after every saturating Xe flash, indicating the accessibility of specific surface binding sites on the immobilized RC, for the external electron donor. This new type of bio-nanomaterial is considered as an excellent model for new generation applications of silicon-based electronics and biological redox systems.

Keywords:
Porous silicon functionalization; Peptide; Photosynthetic reaction center; Nanomaterial; Biophotonics