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Open Access Nano Express

Surface enhanced fluorescence of anti-tumoral drug emodin adsorbed on silver nanoparticles and loaded on porous silicon

Margarita Hernandez1, Gonzalo Recio2, Raul J Martin-Palma2, Jose V Garcia-Ramos1, Concepcion Domingo1 and Paz Sevilla13*

Author affiliations

1 Instituto de Estructura de la Materia, IEM-CSIC, Serrano 121, Madrid, 28006, Spain

2 Departamento de Fisica Aplicada, Facultad de Ciencias, UAM, Madrid, 28049, Spain

3 Departamento de Quimica Fisica II, Facultad de Farmacia, UCM, Madrid, 28040, Spain

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Citation and License

Nanoscale Research Letters 2012, 7:364  doi:10.1186/1556-276X-7-364

Published: 2 July 2012

Abstract

Fluorescence spectra of anti-tumoral drug emodin loaded on nanostructured porous silicon have been recorded. The use of colloidal nanoparticles allowed embedding of the drug without previous porous silicon functionalization and leads to the observation of an enhancement of fluorescence of the drug. Mean pore size of porous silicon matrices was 60 nm, while silver nanoparticles mean diameter was 50 nm. Atmospheric and vacuum conditions at room temperature were used to infiltrate emodin-silver nanoparticles complexes into porous silicon matrices. The drug was loaded after adsorption on metal surface, alone, and bound to bovine serum albumin. Methanol and water were used as solvents. Spectra with 1 μm spatial resolution of cross-section of porous silicon layers were recorded to observe the penetration of the drug. A maximum fluorescence enhancement factor of 24 was obtained when protein was loaded bound to albumin, and atmospheric conditions of inclusion were used. A better penetration was obtained using methanol as solvent when comparing with water. Complexes of emodin remain loaded for 30 days after preparation without an apparent degradation of the drug, although a decrease in the enhancement factor is observed. The study reported here constitutes the basis for designing a new drug delivery system with future applications in medicine and pharmacy.

Keywords:
Surface-enhanced fluorescence; Porous Silicon; Emodin; Drug delivery