Table 2 |
||||
| Characterizations of TbADH and NADPH immobilized on Au-( n %)glutananoparticles | ||||
| Glutaraldehyde ( n %) |
Enzyme loading (mg/mL) (%) |
Activity expressed (U/mL) | Specific activity (U/mg) | CNADPH(mM) |
| Free TbADH | - | 0.5 | 12.5 | - |
| Free NADPH | - | - | - | 1.6 |
| 0.3 | 1.8 (88) | 0.23 | 5.8 | 1.2 |
| 0.5 | 1.8 (91) | 0.27 | 6.2 | 1.23 |
| 0.7 | 1.8 (92) | 0.36 | 9.0 | 1.26 |
| 1.0 | 1.9 (96) | 0.35 | 8.7 | 1.25 |
| 1.4 | 1.8 (92) | 0.13 | 2.6 | 1.2 |
| 1.8 | 1.8 (92) | 0.1 | 2.6 | 1.2 |
aThe mean size of Au nanoparticles was 15 nm, and n is the percent of glutaraldehyde solution used in the treatment.
Petkova et al. Nanoscale Research Letters 2012 7:287 doi:10.1186/1556-276X-7-287
