Table 2

Characterizations of TbADH and NADPH immobilized on Au-(n%)glutananoparticles
Glutaraldehyde (n%)

Enzyme loading

(mg/mL) (%)

Activity expressed (U/mL) Specific activity (U/mg) CNADPH(mM)
Free TbADH - 0.5 12.5 -
Free NADPH - - - 1.6
0.3 1.8 (88) 0.23 5.8 1.2
0.5 1.8 (91) 0.27 6.2 1.23
0.7 1.8 (92) 0.36 9.0 1.26
1.0 1.9 (96) 0.35 8.7 1.25
1.4 1.8 (92) 0.13 2.6 1.2
1.8 1.8 (92) 0.1 2.6 1.2

aThe mean size of Au nanoparticles was 15 nm, and n is the percent of glutaraldehyde solution used in the treatment.

Petkova et al.

Petkova et al. Nanoscale Research Letters 2012 7:287   doi:10.1186/1556-276X-7-287

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