Table 1

Characterizations of TbADH and NADPH immobilized on functionalized Ag and Au nanoparticles
Nanoparticles' system

Enzyme loading

(mg/mL) (%)

Activity expressed (U/mL) Specific activity (U/mg) CNADPH, mM
Free TbADH - 0.5 12.5 -
Free NADPH - - - 1.6
Ag-COOHa 2.9 (97) 0.23 5.8 1.28
Au-citr(CTAB)b 2.9 (98) 0.04 1.1 1.2
Au-COOHb 2.9 (98) 0.14 3.5 1.23
Au-COOH(CTAB)b 2.9 (98) 0.13 3.2 1.25

The mean size of the nanoparticles was a45 nm for Ag nanoparticles and b15 nm for all types of Au nanoparticles.

Petkova et al.

Petkova et al. Nanoscale Research Letters 2012 7:287   doi:10.1186/1556-276X-7-287

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