Table 1

Characterizations of TbADH and NADPH immobilized on functionalized Ag and Au nanoparticles
Nanoparticles' system

Enzyme loading

(mg/mL) (%)

Activity expressed (U/mL) Specific activity (U/mg) CNADPH, mM
Free TbADH - 0.5 12.5 -
Free NADPH - - - 1.6
Ag-COOHa 2.9 (97) 0.23 5.8 1.28
Au-citr(CTAB)b 2.9 (98) 0.04 1.1 1.2
Au-COOHb 2.9 (98) 0.14 3.5 1.23
Au-COOH(CTAB)b 2.9 (98) 0.13 3.2 1.25

The mean size of the nanoparticles was a45 nm for Ag nanoparticles and b15 nm for all types of Au nanoparticles.

Petkova et al. Nanoscale Research Letters 2012 7:287   doi:10.1186/1556-276X-7-287

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