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Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles

Soo Youn Lee, Chi Young Ahn, Jiho Lee, Jin Hyung Lee and Jeong Ho Chang*

Author Affiliations

Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801, South Korea

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Nanoscale Research Letters 2012, 7:279  doi:10.1186/1556-276X-7-279

Published: 31 May 2012


Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.

Silica-coated magnetic nanoparticles; Thiol; Mixed protein separation; BSA; LYZ