Open Access Nano Express

Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles

Soo Youn Lee, Chi Young Ahn, Jiho Lee, Jin Hyung Lee and Jeong Ho Chang*

Author Affiliations

Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801, South Korea

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Nanoscale Research Letters 2012, 7:279  doi:10.1186/1556-276X-7-279

Published: 31 May 2012

Abstract

Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.

Keywords:
Silica-coated magnetic nanoparticles; Thiol; Mixed protein separation; BSA; LYZ