Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles
Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801, South Korea
Nanoscale Research Letters 2012, 7:279 doi:10.1186/1556-276X-7-279Published: 31 May 2012
Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.