Prussian blue staining (A) and ICP-OES quantification of intracellular iron contents (B) of RAW267.4 cell incubated with SHU555A or MNCs with average size of 63 nm at different iron concentrations for 1 h. Cell uptake of both iron oxide particles are dose-dependent. However, cell incorporation of the MNCs is significantly higher than that of SHU555A. Bar: 20 μm. Inset (Figure B): Amplification of cell uptake at lower iron concentrations.
Li et al. Nanoscale Research Letters 2012 7:204 doi:10.1186/1556-276X-7-204