Figure 2.

Neuronal marker expression. Neuronal marker, β-tubulin III, expression of hESC cultured on (a) PLO exhibiting long two-dimensional axonal development with lower density, (b) silk scaffolds exhibiting some cell migration along with negligible axonal projections, and (c) silk-CNT scaffolds demonstrating three-dimensional axonal elongation as well as cell migration. Scale bar, 200 μm.