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Preparation of α-mannoside hydrogel and electrical detection of saccharide-protein interactions using the smart gel-modified gate field effect transistor

Yasuhiro Maeda1, Akira Matsumoto1, Yoshiko Miura2 and Yuji Miyahara1*

Author Affiliations

1 Institute of Biomaterials & Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

2 Department of Chemical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

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Nanoscale Research Letters 2012, 7:108  doi:10.1186/1556-276X-7-108

Published: 7 February 2012


The purpose of this study was to detect saccharide-protein interaction capitalizing on the gel-modified field effect transistor [FET]. A lectin-sensitive polymer gel that undergoes volume changes in response to the formation of molecular complex between 'pendant' carbohydrate and a 'target' lectin concanavalin A [Con A] was synthesized. It was revealed that direction and magnitude of the gel response (swelling or deswelling) could be readily designed depending on composition and network density of the gel. The Con A-sensitive polymer gel has shown the ability to transduce the detection of saccharide-protein interactions into electrical signals for FET.

PACS: 87.85.jf, bio-based materials

Saccharide-protein interaction; field effect transistor; potentiometry; soft interface; concanavalin A; stimuli-responsive gel; molecular recognition.