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Characteristics of functionalized nano-hydroxyapatite and internalization by human epithelial cell

Zhao Yan-zhong123, Huang Yan-yan1, Zhu Jun2, Zhu Shai-hong13*, Li Zhi-you2 and Zhou Ke-chao23*

Author Affiliations

1 Medical Experiment Center in the Third Xiangya Hospital, Central South University, Changsha 410013, China

2 State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China

3 Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China

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Nanoscale Research Letters 2011, 6:600  doi:10.1186/1556-276X-6-600

Published: 23 November 2011


Hydroxyapatite is the main inorganic component of biological bone and tooth enamel, and synthetic hydroxyapatite has been widely used as biomaterials. In this study, a facile method has been developed for the fabrication of arginine-functionalized and europium-doped hydroxyapatite nanoparticles (Arg-Eu-HAP). The synthesized nanoparticles characterized by transmission electron microscopy, X-ray diffractometry, Fourier transform infrared, and Zeta potential analyzer. Its biological properties with DNA binding, cell toxicity, cell binding and intracellular distribution were tested by agarose gel electrophoresis assay, flow cytometry, and fluorescence microscope and laser scanning confocal microscope. The synthesized Arg-Eu-HAP could effectively bind DNA without any cytotoxicity and be internalized into the cytoplasm and perinuclear of human lung epithelial cells.

hydroxyapatite nanoparticles; arginine; europium; dope; cellular internalization