Table 2 |
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The values of kon,koff and KD for the Ad/CYP11A1 monomeric and the Ad/CYP11A1 oligomeric complex formation reaction |
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kon × 103 [M-1 s-1] |
koff [s-1] |
KD [μM] |
|
|
|
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Monomeric CYP11A1 |
290 ± 30 |
0.05 ± 0.005 |
0.17 ± 0.015 |
|
Oligomeric CYP11A1 |
420 ± 40 |
0.09 ± 0.009 |
0.21 ± 0.02 |
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|
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Optico-biosensoric experiments were performed using a Biacore 3000 device. Approximately 400 RU of Ad were covalently immobilized on a carboxymethylated dextran matrix. Subsequently, different concentrations of CYP11A1 were passed over the flow cell. KD values were determined using the 1:1 binding mechanism available in the Biacore evaluation software 4.1 |
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Ivanov et al. Nanoscale Res Lett 2011 6:54 doi:10.1007/s11671-010-9809-5 |
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