Table 2

The values of kon,koff and KD for the Ad/CYP11A1 monomeric and the Ad/CYP11A1 oligomeric complex formation reaction

kon × 103 [M-1 s-1]

koff [s-1]

KD [μM]


Monomeric CYP11A1

290 ± 30

0.05 ± 0.005

0.17 ± 0.015

Oligomeric CYP11A1

420 ± 40

0.09 ± 0.009

0.21 ± 0.02


Optico-biosensoric experiments were performed using a Biacore 3000 device. Approximately 400 RU of Ad were covalently immobilized on a carboxymethylated dextran matrix. Subsequently, different concentrations of CYP11A1 were passed over the flow cell. KD values were determined using the 1:1 binding mechanism available in the Biacore evaluation software 4.1

Ivanov et al. Nanoscale Res Lett 2011 6:54   doi:10.1007/s11671-010-9809-5

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