AFM images (a) and the corresponding density of distribution with height (ρ(h)) for AdR/Ad complexes (b); comparison of ρ(h)AdR/Ad vs. normalized distribution densities of individual AdR and Ad (the summarized area under ρ(h)AdR and ρ(h)Ad curves is reduced to 100%) (c); and the sum of normalized distribution densities of individual AdR and Ad (d). Tapping mode. Experimental conditions were as follows: the mixture of 5 μM solutions (10 μl each) of appropriate individual proteins (AdR and Ad) in the 50 mM KP, pH 7.4, was incubated for 10 min, diluted 2.5 times with the same buffer, and a 5-μl portion of the mixture was immediately placed onto mica, T = 25°C. Arrows (1) indicate the images of AdR and Ad monomers. Arrows (2) indicate the AdR/Ad images.
Ivanov et al. Nanoscale Res Lett 2011 6:54 doi:10.1007/s11671-010-9809-5