AFM images of the objects (a) and the corresponding density of distribution with height (ρ(h)Ad + CYP11A1) for Ad + CYP11A1 mixture (b); comparison of ρ(h)Ad + CYP11A1 vs. normalized distribution densities of individual Ad and CYP11A1 monomers (summarized area under ρ(h)Ad and ρ(h)CYP11A1 curves is reduced to 100%) (c); differential curve (Δρ) between ρ(h)Ad + CYP11A1 and the sum of normalized distribution densities of individual Ad and CYP11A1 (d). Tapping mode. Experimental conditions were as follows: the mixture of 5 μM solutions (10 μl each) of appropriate individual proteins (monomeric CYP11A1, containing 1% Emulgen 913, and Ad) in 50 mM KP, pH 7.4, was incubated for 10 min, diluted 2.5 times with the same buffer, and a 5-μl portion of the mixture was immediately placed onto mica, T = 25°C.
Ivanov et al. Nanoscale Res Lett 2011 6:54 doi:10.1007/s11671-010-9809-5